CD38/CD19: a lipid raft-dependent signaling complex in human B cells

被引:93
|
作者
Deaglio, Silvia [1 ]
Vaisitti, Tiziana
Billington, Richard
Bergui, Luciana
Omede, Paola
Genazzani, Armando A.
Malavasi, Fabio
机构
[1] Univ Turin, Sch Med, Res Ctr Expt Med CeRMS, Dept Genet Biol & Biochem, Turin, Italy
[2] Univ Piemonte Orientale, Drug & Food Biotechnol Ctr, Dipartimento Sci Chim AFF, Novara, Italy
[3] Univ Turin, Sch Med, S Giovanni Battista Hosp, Dept Med & Expt Oncol Div Hematol, Turin, Italy
关键词
D O I
10.1182/blood-2006-12-061812
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
The present work deals with the mechanisms of signal transduction mediated via CD38 in normal and neoplastic human B lymphocytes. The results indicate that CD38 is a receptor and that CD38-mediated signals are tightly regulated at 3 distinct levels. The first concerns the structural organization of CD38, which is clearly divided into monomeric and dimeric forms. The second level of regulation is based on the dynamic localization of CD38 molecules in lipid microdomains Introduction within the plasma membrane. Lateral associations with other proteins, namely with the CD19/CD81 complex, determine the third level of control. Raft localization and association with the CD19 complex are prerequisites for C1338-mediated signals in tonsillar B cells and in continuous lines. Lastly, the results indicate that lipid microdomain disruption and silencing of CD1 9 directly impacts on CD38's ability to mediate Ca2+ fluxes, while leaving its surface expression unchanged. CD38 is also an enzyme capable of producing several calcium-mobilizing metabolites including cyclic adenosine diphosphate ribose (cADPR). Our inability to identify a correlation between the production of cADPR and the receptorial functions support the hypothesis that CD38 is a pleiotropic molecule whose behavior as a receptor is independent from its enzymatic activity.
引用
收藏
页码:5390 / 5398
页数:9
相关论文
共 50 条
  • [1] MHC class II/CD38/CD9: a lipid-raft-dependent signaling complex in human monocytes
    Zilber, MT
    Setterblad, N
    Vasselon, T
    Doliger, C
    Charron, D
    Mooney, N
    Gelin, C
    [J]. BLOOD, 2005, 106 (09) : 3074 - 3081
  • [2] Role of CD38 in human B cells: evidence of a functional and physical association with CD19
    Deaglio, S
    Vaisitti, T
    Malavasi, F
    [J]. FASEB JOURNAL, 2005, 19 (04): : A952 - A952
  • [3] The CD19/CD81 complex physically interacts with CD38 but is not required to induce proliferation in mouse B lymphocytes
    Vences-Catalan, Felipe
    Rajapaksa, Ranjani
    Levy, Shoshana
    Santos-Argumedo, Leopoldo
    [J]. IMMUNOLOGY, 2012, 137 (01) : 48 - 55
  • [4] Role of human CD38 in B cell signaling
    Vaisitti, Tiziana
    Deaglio, Silvia
    Aydin, Semra
    Malavasi, Fabio
    [J]. MOLECULAR MEDICINE, 2006, 12 : S25 - S25
  • [5] The lack of the CD38 and CD19 genes impact in the development of splenic immature B cells from mice
    Gallardo-Hernandez, C. A.
    Romero-Ramirez, H.
    Rodriguez-Alba, J. C.
    [J]. EUROPEAN JOURNAL OF IMMUNOLOGY, 2019, 49 : 335 - 335
  • [6] CD38 is functionally dependent on the TCR/CD3 complex in human T cells
    Morra, M
    Zubiaur, M
    Terhorst, C
    Sancho, J
    Malavasi, F
    [J]. FASEB JOURNAL, 1998, 12 (07): : 581 - 592
  • [7] CD38 ligation in human B cell progenitors triggers tyrosine phosphorylation of CD19 and association of CD19 with lyn and phosphatidylinositol 3-kinase
    Kitanaka, A
    Ito, C
    CoustanSmith, E
    Campana, D
    [J]. JOURNAL OF IMMUNOLOGY, 1997, 159 (01): : 184 - 192
  • [8] ANTI-CD20 ANTIBODY-MEDIATED APOPTOSIS OF B CELLS IS A LIPID RAFT-DEPENDENT PROCESS
    Pers, Jacques-Oliver
    Hammadi, Mariam
    Dalbies, Florence
    Tempescul, Adrian
    Berthou, Christian
    Bordron, Anne
    Youinou, Pierre
    [J]. ANNALS OF THE RHEUMATIC DISEASES, 2011, 70 : A73 - A73
  • [9] Individual CD34(+)CD38(low)CD19(-)CD10(-) progenitor cells from human cord blood generate B lymphocytes and granulocytes
    Berardi, AC
    Meffre, E
    Pflumio, F
    Katz, A
    Vainchenker, W
    Schiff, C
    Coulombel, L
    [J]. BLOOD, 1997, 89 (10) : 3554 - 3564
  • [10] Role of CD38 in human B cells: Evidence of a functional and physical association with CD19.
    Deaglio, S
    Vaisitti, T
    Malavasi, F
    [J]. CLINICAL IMMUNOLOGY, 2005, 115 : S6 - S7