Mediator lipidomics by liquid chromatography-tandem mass spectrometry

被引：4
|
作者：
Kita, Yoshihiro [1 ,2 ]
Tokuoka, Suzumi M. [1 ]
Shimizu, Takao [1 ,3 ]
机构：
[1] Univ Tokyo, Grad Sch Med, Dept Lipid, Bunkyo Ku, 7-3-1 Hongo, Tokyo 1130033, Japan
[2] Univ Tokyo, Grad Sch Med, Life Sci Core Facil, Bunkyo Ku, 7-3-1 Hongo, Tokyo 1130033, Japan
[3] Natl Ctr Global Hlth & Med, Dept Lipid Signaling, Res Inst, Shinjuku Ku, 1-21-1 Toyama, Tokyo 1628655, Japan
来源：
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS | 2017年 / 1862卷 / 08期
关键词：
Mass spectrometer; Quantification; Eicosanoids; Cross-platform; PLATELET-ACTIVATING-FACTOR; MICROWAVE IRRADIATION; PROSTAGLANDINS; LEUKOTRIENES; PURIFICATION; EICOSANOIDS; EXTRACTION;
D O I：
10.1016/j.bbalip.2017.03.008
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Comprehensive quantitative analysis of lipid mediators using liquid chromatography-tandem mass spectrometry is an effective strategy in the elucidation of disease mechanisms; but technically, it has been and is still a great challenge to achieve reliable datasets that cover variety of lipid metabolites contained at trace levels in complex biological matrices. In this opinion article, we introduce our experiences in developing lipid mediator profiling systems, and deliver some comments on limitations of current methodology.
引用
收藏
页码：777 / 781
页数：5
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