A novel methymycin analog, 12-ketomethymycin N-oxide, produced by the heterologous expression of the large pikromycin/methymycin biosynthetic gene cluster of Streptomyces sp. AM4900

被引：0

作者：

Ueoka, Reiko ^{[1
]}

Hashimoto, Junko ^{[2
]}

Kozone, Ikuko ^{[2
]}

Hashimoto, Takuya ^{[1
]}

Kudo, Kei ^{[1
]}

Kagaya, Noritaka ^{[1
]}

Suenaga, Hikaru ^{[1
]}

Ikeda, Haruo ^{[3
]}

Shin-ya, Kazuo ^{[1
,4
]}

机构：

[1] Natl Inst Adv Ind Sci & Technol, Tokyo, Japan

[2] Japan Biol Informat Consortium JBIC, Tokyo, Japan

[3] Kitasato Univ, Kitasato Inst Life Sci, Sagamihara, Kanagawa, Japan

[4] Univ Tokyo, Biotechnol Res Ctr, Tokyo, Japan

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2021年 / 85卷 / 04期

关键词：

actinomycete; heterologous expression; isolation; structure elucidation; 16-MEMBERED MACROLIDE ANTIBIOTICS; AVERMITILIS; PIKROMYCIN; GENOME; SYNTHASE; CLONING;

D O I：

10.1093/bbb/zbaa111

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A novel methymycin analog, 12-ketomethymycin N-oxide, was produced by the heterologous expression of the pikromycin/methymycin biosynthetic gene cluster of Streptomyces sp. AM4900 together with 12-ketomethymycin, which was only isolated by the biotransformation of the synthetic intermediate before. Their structures were determined by the spectroscopic data and the chemical derivatization. 12-Ketomethymycin showed a weak cytotoxicity against SKOV-3 and Jurkat cells, although its N-oxide analog did not show any activity. Both showed no antibacterial activities against Escherichia coli and Micrococcus luteus.

引用

页码：890 / 894

页数：5

共 4 条

[1] Neothioviridamide, a Polythioamide Compound Produced by Heterologous Expression of a Streptomyces sp. Cryptic RiPP Biosynthetic Gene Cluster
Kawahara, Teppei
Izumikawa, Miho
Kozone, Ikuko
Hashimoto, Junko
Kagaya, Noritaka
Koiwai, Hanae
Komatsu, Mamoru
Fujie, Manabu
Sato, Noriyuki
Ikeda, Haruo
Kazuo, Shin-ya
JOURNAL OF NATURAL PRODUCTS, 2018, 81 (02): : 264 - 269
[2] Novel macrolactam compound produced by the heterologous expression of a large cryptic biosynthetic gene cluster of Streptomyces rochei IFO12908
Hashimoto, Takuya
Kozone, Ikuko
Hashimoto, Junko
Ueoka, Reiko
Kagaya, Noritaka
Fujie, Manabu
Sato, Noriyuki
Ikeda, Haruo
Shin-ya, Kazuo
JOURNAL OF ANTIBIOTICS, 2020, 73 (03): : 171 - 174
[3] Novel macrolactam compound produced by the heterologous expression of a large cryptic biosynthetic gene cluster of Streptomyces rochei IFO12908
Takuya Hashimoto
Ikuko Kozone
Junko Hashimoto
Reiko Ueoka
Noritaka Kagaya
Manabu Fujie
Noriyuki Sato
Haruo Ikeda
Kazuo Shin-ya
The Journal of Antibiotics, 2020, 73 : 171 - 174
[4] Cloning, sequencing and heterologous expression of the medermycin biosynthetic gene cluster of Streptomyces sp.: AM-7161:: towards comparative analysis of the benzoisochromanequinone gene clusters
Ichinose, K
Ozawa, M
Itou, K
Kunieda, K
Ebizuka, Y
MICROBIOLOGY-SGM, 2003, 149 : 1633 - 1645

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