Effects of water deficit stress on growth, water relations and osmolyte accumulation in Medicago truncatula and M. laciniata populations

The effects of water stress were investigated in two Tunisian Medicago truncatula populations collected from arid (Mt-173) and sub-humid (Mt-664) climates and two Tunisian M. laciniata populations originating from arid (MI-173) and semi-arid (MI-345) regions. After a pre-treatment phase (24 days after sowing, DAS) of watering at 100% of field capacity (FC), the plants were either irrigated at 100% FC or at only 33% FC. After 12 days of treatment (36 DAS), one lot of dehydrated plants was rewatered at 100% FC. A final harvest was carried out after 24 days of treatment (48 DAS). Measured parameters were total dry weight (TOW), root shoot ratio (RSR), leaf relative water content (RWC), osmotic potential (OP), photosynthetic parameters (CO2 net assimilation A, stomatal conductance g(s) and transpiration E), malondialdehyde (MDA) concentration and leaf contents in inorganic (Na+ and K+) and organic solutes (proline and soluble sugars). Under water deficit conditions, compared to M. laciniata, M. truncatula populations showed a higher reduction in TDW, A, g(s) and RWC associated with a higher increase in MDA concentration. Thus, the relative tolerance of M. laciniata populations to water shortage would be related to their lower intrinsic growth rate and stomatal control of gas exchange. TDW, A, g(s), E and RWC were more decreased by water deficit in MI-345 than in MI-173. Drought tolerance of MI-173 was found to be associated with a more pronounced decrease of OP and a lower reduction in RWC due to the accumulation of solutes such as proline, soluble sugars and K+. In addition, MI-173 showed the highest water use efficiency values (WUE) and the lowest MDA concentrations under water deficit stress. (C) 2009 Academie des sciences. Published by Elsevier Masson SAS. All rights reserved.