Acid mine drainage removal by mixed bacteria culture of Pseudomonas aeruginosa and Brevibacterium sp.

Acid mine drainage is a problem faced by the mining industry in Indonesia has a negative impact on the environment because it is very acidic and contains heavy metals. This bioremediation research to determine the ability of a mixed bacteria culture of Pseudomonas aeruginosa and Brevibacterium sp. in removal acid mine water. The study began with an allowance test in liquid media using artificial growth media Stone Mineral Salt solution (SMSs) with the tested parameters namely contact time (hours) and concentration of acid mine water pollutants (% v/v). Parameter variation consists of contact time (hours) 24 to 240 and concentration of acid mine pollutant water (% v/v) 10 to 25. Mixed culture are resistant to the presence of acid mine water as a substrate that is xenobiotic because no inhibition zones are formed around paper discs that have been exposed to acid mine pollutant water and can grow on Nutrient Agar (NA) media added with acid mine pollutants. The pH of the liquid media increased from 2.14 to 5,87 with optimum contact time 144 hours with a temperature of 30 degrees C and optimum pollutant concentration 10%.