Detection and quantification of macrolide resistance mutations at positions 2058 and 2059 of the 23S rRNA gene by pyrosequencing

被引:47
|
作者
Haanperä, M
Huovinen, P
Jalava, J
机构
[1] Natl Publ Hlth Inst, Human Microbial Ecol Lab, FI-20520 Turku, Finland
[2] Natl Publ Hlth Inst, Antimicrobial Res Lab, FI-20520 Turku, Finland
关键词
D O I
10.1128/AAC.49.1.457-460.2005
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
A pyrosequencing method for detection and quantification of macrolide resistance mutations at positions 2058 and 2059 (Escherichia coli numbering) of the 23S rRNA gene is described. The method was developed and tested for Streptococcus pneumoniae, Streptococcus pyogenes, Mycobacterium avium, Campylobacter jejuni, and Haemophilus influenzae.
引用
收藏
页码:457 / 460
页数:4
相关论文
共 50 条
  • [1] Mutation at the position 2058 of the 23S rRNA as a cause of macrolide resistance in Streptococcus pyogenes
    Jalava J.
    Vaara M.
    Huovinen P.
    [J]. Annals of Clinical Microbiology and Antimicrobials, 3 (1)
  • [2] The structural basis of macrolide-ribosome binding assessed using mutagenesis of 23 S rRNA positions 2058 and 2059
    Pfister, P
    Jenni, S
    Poehlsgaard, J
    Thomas, A
    Douthwaite, S
    Ban, N
    Böttger, EC
    [J]. JOURNAL OF MOLECULAR BIOLOGY, 2004, 342 (05) : 1569 - 1581
  • [3] 23S rRNA Gene Mutations Contributing to Macrolide Resistance in Campylobacter jejuni and Campylobacter coli
    Ladely, Scott R.
    Meinersmann, Richard J.
    Englen, Mark E.
    Fedorka-Cray, Paula J.
    Harrison, Mark A.
    [J]. FOODBORNE PATHOGENS AND DISEASE, 2009, 6 (01) : 91 - 98
  • [4] Rapid detection, by PCR and reverse hybridization, of mutations in the Helicobacter pylori 23S rRNA gene, associated with macrolide resistance
    van Doorn, LJ
    Debets-Ossenkopp, YJ
    Marais, A
    Sanna, R
    Mégraud, F
    Kusters, JG
    Quint, WGV
    [J]. ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1999, 43 (07) : 1779 - 1782
  • [5] Detection of A2058G and A2059G on the 23S rRNA Gene by Multiplex Nested PCR to Identify Treponema pallidum Resistance to Azithromycin in Indonesia
    Rosana, Yeva
    Yasmon, Andi
    Indriatmi, Wresti
    Effendi, Ida
    Kusumawati, Raden Lia
    Rowawi, Rasmia
    Sudigdoadi, Sunarjati
    Pradini, Gita Widya
    Wiraguna, Anak Agung Gde Putra
    Puspawati, Ni Made Dwi
    Kusumawaty, Maryam
    Massi, Muhammad Nasrum
    [J]. JAPANESE JOURNAL OF INFECTIOUS DISEASES, 2022, 75 (04) : 355 - 360
  • [6] Increases in the Macrolide Resistance of Mycoplasma genitalium and the Emergence of the A2058T Mutation in the 23S rRNA Gene: Clonal Spread?
    Pineiro, Luis
    Idigoras, Pedro
    Arrastia, Maitane
    Manzanal, Ayla
    Ansa, Inigo
    Cilla, Gustavo
    [J]. ANTIBIOTICS-BASEL, 2022, 11 (11):
  • [7] Direct assessment of possible mutations in the 23S rRNA gene encoding macrolide resistance in Chlamydia trachomatis
    van Niekerk, J. M.
    van Loo, I. H. M.
    Lucchesi, M.
    Morre, S. A.
    Hoebe, C. J. P. A.
    Dukers-Muijrers, N. H. T. M.
    Wolffs, P. F. G.
    [J]. PLOS ONE, 2022, 17 (05):
  • [8] Single and dual mutations at positions 2058, 2503 and 2504 of 23S rRNA and their relationship to resistance to antibiotics that target the large ribosomal subunit
    Li, Bei-Bei
    Wu, Cong-Ming
    Wang, Yang
    Shen, Jian-Zhong
    [J]. JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 2011, 66 (09) : 1983 - 1986
  • [9] Macrolide resistance conferred by base substitutions in 23S rRNA
    Vester, B
    Douthwaite, S
    [J]. ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2001, 45 (01) : 1 - 12
  • [10] A Novel SimpleProbe PCR Assay for Detection of Mutations in the 23S rRNA Gene Associated with Macrolide Resistance in Mycoplasma genitalium in Clinical Samples
    Gosse, Marianne
    Lysvand, Hilde
    Pukstad, Brita
    Nordbo, Svein Arne
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2016, 54 (10) : 2563 - 2567