Multidimensional counter-current chromatographic system and its application

被引：60
|
作者：
Yang, FQ
Quan, J
Zhang, TY
Ito, Y
机构：
[1] NHLBI, Biophys Chem Lab, NIH, Bethesda, MD 20892 USA
[2] Beijing Inst New Technol Applicat, Beijing 100035, Peoples R China
来源：
JOURNAL OF CHROMATOGRAPHY A | 1998年 / 803卷 / 1-2期
关键词：
counter-current chromatography; multidimensional separation; Ginkgo biloba; Hippophae rhamnoides; isorhamnetin; kaempferol; quercetin; flavone aglycones; aglycones;
D O I：
10.1016/S0021-9673(97)01273-9
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A multidimensional counter-current chromatographic system was set up for the first time with two sets of high-speed counter-current chromatography instruments. This system was successfully applied to the preparative separation of isorhamnetin, kaempferol and quercetin from crude flavone aglycones of Ginkgo biloba L. and Hippophae rhamnoides L. with a two-phase solvent system composed of chloroform-methanol-water (4:3:2, v/v/v). (C) 1998 Elsevier Science B.V.
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页码：298 / 301
页数：4
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