Genetic variability of E6 and E7 genes of human papillomavirus type 58 in Jingzhou, Hubei Province of central China

被引:11
|
作者
Yang, Zhiping [1 ]
Zhang, Chunlin [1 ]
Luo, Ping [1 ]
Ye, Mengxia [1 ]
Gong, Quan [2 ]
Mei, Bing [1 ]
机构
[1] Yangtze Univ, Jingzhou Hosp, Dept Lab Med, Jingzhou 434020, Peoples R China
[2] Yangtze Univ, Sch Med, Dept Immunol, Jingzhou, Peoples R China
关键词
Human papillomavirus type 58 (HPV-58); Variants; Epitope; Phylogenetic analysis; Selection pressure; CERVICAL-CANCER; RISK; VARIANTS; WOMEN; CARCINOGENESIS; NEOPLASIA; SICHUAN; GENOME;
D O I
10.1186/s12985-022-01801-6
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Background Cervical cancer is a common malignant tumor in women, with a high mortality rate, has great harm to women's health. Long-term and persistent infection of high-risk human papillomavirus (HR-HPV) is the main reason of the occurrence and development of cervical cancer. Methods The infection rate of HPV-58 is higher in the Jingzhou area. In this study, 172 complete HPV-58 E6-E7 sequences were amplified by polymerase chain reaction (PCR), the amplified products were sequenced, and the gene variations of HPV-58 E6-E7 were analyzed. A Neighbor-Joining phylogenetic tree was constructed by MEGA 11. The secondary structure of E6 and E7 protein was investigated. PAML X was used to analyze the selective pressure. The B cell epitopes of E6 and E7 proteins in HPV-58 were predicted by ABCpred server. Results In E6 sequences, 10 single nucleotide variants were observed, including 7 synonymous and 3 non-synonymous variants. In E7 sequences, 12 single nucleotide variants were found, including 3 synonymous variants and 9 non-synonymous variants. There are 5 novel variants. The phylogenetic analysis showed that all the E6-E7 sequences were distributed in A lineage. No positively selected site was found in E6 sequence, but G63 in E7 sequences was identified as positively selected site. Some amino acid substitutions affected multiple B cell epitopes. Conclusion Various E6 and E7 mutational data may prove useful for development of better diagnostic and vaccines for the region of Jingzhou, Hubei province of central China.
引用
收藏
页数:8
相关论文
共 50 条
  • [1] Genetic variability of E6 and E7 genes of human papillomavirus type 58 in Jingzhou, Hubei Province of central China
    Zhiping Yang
    Chunlin Zhang
    Ping Luo
    Mengxia Ye
    Quan Gong
    Bing Mei
    [J]. Virology Journal, 19
  • [2] Genetic variability and oncogenic risk association of human papillomavirus type 58 E6 and E7 genes in Taizhou area, China
    Yu, Jun-Hui
    Shi, Wei-Wu
    Zhou, Mei-Ying
    Liu, Jia-Mei
    Han, Qiu-Yue
    Xu, Hui-Hui
    [J]. GENE, 2019, 686 : 171 - 176
  • [3] Genetic variation of E6 and E7 genes of human papillomavirus type 16 from central China
    Li, Ting
    Yang, Zhiping
    Zhang, Chunlin
    Wang, Sutong
    Mei, Bing
    [J]. VIROLOGY JOURNAL, 2023, 20 (01)
  • [4] Genetic variation of E6 and E7 genes of human papillomavirus type 16 from central China
    Ting Li
    Zhiping Yang
    Chunlin Zhang
    Sutong Wang
    Bing Mei
    [J]. Virology Journal, 20
  • [5] Genetic variability of human papillomavirus type 18 based on E6, E7 and L1 genes in central China
    Li, Ting
    Yang, Zhiping
    Luo, Ping
    Yang, Yang
    Lin, Zicong
    Mei, Bing
    [J]. VIROLOGY JOURNAL, 2024, 21 (01)
  • [6] Genetic Variability in E6 and E7 Oncogenes from Human Papillomavirus Type 58 in Mexican Women
    Conde-Ferraeza, Laura
    Pacheco-Arjona, Ramon
    Canul, Cinthia Novelo
    Gomez-Carballo, Jesus
    Humberto Ramirez-Prado, Jorge
    Ayora-Talavera, Guadalupe
    del Refugio Gonzalez-Losa, Maria
    [J]. INTERVIROLOGY, 2017, 60 (06) : 235 - 246
  • [7] Genetic variation of E6 and E7 genes of human papillomavirus 52 from Central China
    Li, Shuo
    Ye, Mengxia
    Chen, Yonglin
    Gong, Quan
    Mei, Bing
    [J]. JOURNAL OF MEDICAL VIROLOGY, 2021, 93 (06) : 3849 - 3856
  • [8] Genetic variability of HPV-58 E6 and E7 genes in Southwest China
    Yang, Lijuan
    Yang, Hongying
    Chen, Junying
    Huang, Xinwei
    Pan, Yue
    Li, Duo
    Ding, Xiaojie
    Wu, Kun
    Shi, Xinan
    Fu, Juanjuan
    Shi, Haijing
    Ma, Shaohui
    Sun, Qiangming
    [J]. INFECTION GENETICS AND EVOLUTION, 2014, 21 : 395 - 400
  • [9] Genetic variability and lineage phylogeny of human papillomavirus type 45 based on E6 and E7 genes in Southwest China
    Cui, Fangying
    Zhang, Zhuo
    Xu, Jianju
    Ding, Xianping
    Mu, Xuemei
    Wan, Qiuling
    Tan, Liping
    Fang, Liyuan
    Chen, Zuyi
    [J]. VIRUS RESEARCH, 2018, 255 : 85 - 89
  • [10] Genetic variability of human papillomavirus type 39 based on E6, E7 and L1 genes in Southwest China
    Jiaoyu He
    Tianjun Li
    Youliang Wang
    Zhilin Song
    Qiufu Li
    Yiran Liu
    Yanru Cui
    Siyu Ma
    Junhang Deng
    Xia Wei
    Xianping Ding
    [J]. Virology Journal, 18