Cohnella saccharovorans sp nov., isolated from ginseng soil

A novel bacterial strain, CJ22(T), was isolated from soil of a ginseng field located in Anseong, Korea. Cells of strain CJ22(T) were aerobic, Gram-stain-positive, endospore-forming, motile, oxidase-and catalase-positive and rod-shaped. The isolate grew optimally at pH 7 and 30 degrees C. Phylogenetic analysis based on the 16S rRNA gene sequence revealed that strain CJ22(T) belonged to the genus Cohnella, displaying highest sequence similarity of 97.3 % with Cohnella panacarvi Gsoil 349(T). DNA-DNA relatedness between strain CJ22(T) and its closest relative was 35.5 % (reciprocal value, 23.8 %). The phenotypic features of strain CJ22(T) also distinguished it from related species of the genus Cohnella. The diagnostic diamino acid in the cell-wall peptidoglycan was meso-diaminopimelic acid. The major isoprenoid quinone was menaquinone MK-7 and the major polar lipids were phosphatidylglycerol, phosphatidylethanolamine, diphosphatidylglycerol, lysyl-phosphatidylglycerol, two unidentified phospholipids and two unidentified aminophospholipids. The predominant cellular fatty acids of strain CJ22(T) were anteiso-C-15 : 0, iso-C-16 : 0 and C-16 : 0. The DNA G+C content was 63.1 mol%. Based on data from this polyphasic taxonomic study, strain CJ22(T) is considered to represent a novel species of the genus Cohnella, for which the name Cohnella saccharovorans sp. nov. is proposed. The type strain is CJ22(T) (=KACC 17501(T) =JCM 19227(T)).