Cleavage of focal adhesion kinase (FAK) is essential in adipocyte differentiation

被引：33
|
作者：
Li, Jing-Jing [1 ]
Xie, Dong [1 ]
机构：
[1] Chinese Acad Sci, Shanghai Inst Biol Sci, Mol Oncol Lab, Inst Nutr Sci,Grad Sch, Shanghai 200031, Peoples R China
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2007年 / 357卷 / 03期
基金：
中国国家自然科学基金;
关键词：
focal adhesion kinase; calpain; cleavage; adipocyte differentiation;
D O I：
10.1016/j.bbrc.2007.03.184
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
During adipocyte differentiation, the cells experience dramatic alterations in morphology, motility and cell-ECM contact. Focal adhesion kinase (pp125FAK), a widely expressed non-receptor tyrosine kinase in integrin signaling, has been reported to participate in these events in various cells. Utilizing 3T3-L1 cells and primary rat preadipocytes, we explored the role of FAK in adipocyte differentiation. Gradual cleavage of FAK was demonstrated during adipcoyte differentiation, both in vitro and in vivo. This cleavage of FAK was mediated by calpain. Inhibition of calpain activity resulted in the rescue of FAK degradation, accompanied with the disturbance of final maturation of adipocyte. Our study revealed that FAK participated in adipocyte differentiation, and its cleavage by calpain was required to fulfill the final maturation of adipocytes. (c) 2007 Elsevier Inc. All rights reserved.
引用
收藏
页码：648 / 654
页数：7
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