Chemo-enzymatic synthesis of 13C- and 19F-labeled uridine-5′-triphosphate for RNA NMR probing

被引：6

作者：

Taiwo, Kehinde M. ^{[1
]}

Becette, Owen B. ^{[1
]}

Zong, Guanghui ^{[1
]}

Chen, Bin ^{[1
]}

Zavalij, Peter Y. ^{[1
]}

Dayie, Theodore Kwaku ^{[1
]}

机构：

[1] Univ Maryland, Dept Chem & Biochem, Ctr Biomol Struct & Org, College Pk, MD 20742 USA

来源：

MONATSHEFTE FUR CHEMIE | 2021年 / 152卷 / 04期

关键词：

Nucleotides; Nucleic acids; Isotopic labeling; Spectroscopy;

D O I：

10.1007/s00706-021-02757-z

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Solution NMR of the magnetically active fluorine-19 (F-19) isotope offers tremendous advantages in the study of RNA structure and conformational changes. However, exploiting these advantages requires the synthesis of fluorinated RNA-building blocks, and this comes with certain challenges in overall yield of nucleotides, purity, and cost. Here, we detail a scalable and reliable method to produce [1 ',5-C-13(2), 5-F-19, 6-H-2]- and [1 ',5-C-13(2), 5-F-19, 6-H-2, 1,3-N-15(2)]-5-fluorouracil-5 '-triphosphate. We further present the steps employed to characterize the labeled nucleotide before its incorporation into RNAs with relevant biological activities.

引用

页码：441 / 447

页数：7

共 16 条

[1] Chemo-enzymatic synthesis of 13C- and 19F-labeled uridine-5′-triphosphate for RNA NMR probing
Kehinde M. Taiwo
Owen B. Becette
Guanghui Zong
Bin Chen
Peter Y. Zavalij
Theodore Kwaku Dayie
[J]. Monatshefte für Chemie - Chemical Monthly, 2021, 152 : 441 - 447
[2] Site-Specific Labeling of RNAs with Modified and 19F-Labeled Nucleotides by Chemo-Enzymatic Synthesis
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