The Methods of Choice for Extracellular Vesicles (EVs) Characterization

被引：397
|
作者：
Szatanek, Rafal [1 ]
Baj-Krzyworzeka, Monika [1 ]
Zimoch, Jakub [2 ]
Lekka, Malgorzata [3 ]
Siedlar, Maciej [1 ]
Baran, Jarek [1 ]
机构：
[1] Jagiellonian Univ, Inst Paediat, Dept Clin Immunol, Med Coll, PL-30663 Krakow, Poland
[2] Univ Childrens Hosp Zurich, Dept Surg, Tissue Biol Res Unit, CH-8032 Zurich, Switzerland
[3] Polish Acad Sci, Inst Nucl Phys, Dept Res Biophys Microstruct, PL-31342 Krakow, Poland
来源：
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2017年 / 18卷 / 06期
关键词：
extracellular vesicles (EVs); exosomes; microvesicles (MVs); flow cytometry; dynamic light scattering (DLS); stimulated emission depletion microscopy (STED); nanoparticle tracking analysis (NTA); transmission electron microscopy (TEM); cryo-electron microscopy (Cryo-EM); atomic force microscopy (AFM); ATOMIC-FORCE MICROSCOPY; NANOPARTICLE TRACKING ANALYSIS; PARTICLE-SIZE DISTRIBUTION; DYNAMIC LIGHT-SCATTERING; PLATELET-DERIVED MICROPARTICLES; FLOW-CYTOMETRY; CIRCULATING MICROPARTICLES; ELECTRON-MICROSCOPY; MEMBRANE-VESICLES; STED MICROSCOPY;
D O I：
10.3390/ijms18061153
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
In recent years, extracellular vesicles (EVs) have become a subject of intense study. These membrane-enclosed spherical structures are secreted by almost every cell type and are engaged in the transport of cellular content (cargo) from parental to target cells. The impact of EVs transfer has been observed in many vital cellular processes including cell-to-cell communication and immune response modulation; thus, a fast and precise characterization of EVs may be relevant for both scientific and diagnostic purposes. In this review, the most popular analytical techniques used in EVs studies are presented with the emphasis on exosomes and microvesicles characterization.
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页数：18
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