Identification of a New Giant Emrbryo Allele, and Integrated Transcriptomics and Metabolomics Analysis of Giant Embryo Development in Rice

被引:7
|
作者
Hu, Zejun [1 ,2 ]
Xiong, Qiangqiang [3 ]
Wang, Kai [1 ]
Zhang, Lixia [1 ,2 ]
Yan, Ying [1 ]
Cao, Liming [1 ,2 ]
Niu, Fuan [1 ]
Zhu, Jinyan [3 ]
Hu, Jinlong [3 ]
Wu, Shujun [1 ,2 ]
机构
[1] Shanghai Acad Agr Sci, Rice Res Ctr, Crop Breeding & Cultivat Res Inst, Shanghai, Peoples R China
[2] Shanghai Agr Prod Preservat Proc Engn Technol Res, Shanghai, Peoples R China
[3] Yangzhou Univ, Coinnovat Ctr Modern Prod Technol Grain Crops, Jiangsu Key Lab Crop Genet & Physiol, Minist Agr,Innovat Ctr Rice Cultivat Technol Yang, Yangzhou, Jiangsu, Peoples R China
来源
关键词
giant embryo; map-based cloning; metabolomics; transcriptomics; metabolic pathway; EXPRESSION ANALYSIS; AUXIN BIOSYNTHESIS; GRAIN-YIELD; GENE; METABOLISM; MUTANT; OMICS; SIZE; QTL;
D O I
10.3389/fpls.2021.697889
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Rice embryos are rich in high-quality protein, lipid, vitamins and minerals, representing the most important nutritional part of brown rice. However, the molecular mechanism of rice embryo development is poorly understood. In this study, two rice cultivars with contrasting embryo size (the giant embryo cultivar Dapeimi and the normal embryo cultivar 187R) were used to explore excellent genes controlling embryo size, and the developed near-isogenic lines (NILs) (NIL-D, which has the giant embryo phenotype, and its matching line, NIL-X) were used to explore transcript and metabolic properties in the earlier maturation stage of giant embryo development under natural conditions. The map-based cloning results demonstrated that Dapeimi is a novel allelic mutant of the rice GIANT EMBRYO (GE) gene, and the functional mutation site is a single cytosine deletion in the exon1. A total of 285 differentially accumulated metabolites (DAMs) and 677 differentially expressed genes (DEGs) were identified between NIL-D and NIL-X. The analysis of DAMs indicated that plants lacking GE mainly promoted energy metabolism, amino acid metabolism, and lipid metabolism pathways in the rice embryo. Pearson correlation coefficient showed that 300 pairs of gene-metabolites were highly correlated. Among them, OsZS_02G0528500 and OsZS_12G0013700 were considered to be key genes regulating L-Aspartic acid and L-Tryptophan content during rice giant embryo development, which are promising to be good candidate genes to improve rice nutrition. By analyzing rice embryo development through a combination of strategies, this research contributes to a greater understanding of the molecular mechanism of rice embryo development, and provides a theoretical foundation for breeding high-nutrition varieties.
引用
收藏
页数:17
相关论文
共 26 条
  • [1] Identification of the biochemical characteristics of developing giant embryo rice grains using non-targeted metabolomics
    Zhao, Guo-Chao
    Zhang, Ying-Xia
    Sun, Shuang-Yan
    Xie, Mi-Xue
    Hu, Chao-Yang
    Shi, Ya-Qiong
    Shi, Jian-Xin
    Li, Jian-Yue
    JOURNAL OF CEREAL SCIENCE, 2019, 85 : 70 - 76
  • [2] Molecular Characterization and Physico-Chemical Analysis of a New Giant Embryo Mutant Allele (get) in Rice (Oryza sativa L.)
    Park, Dong-Soo
    Park, Soo-Kwon
    Lee, Bong-Chun
    Song, Song-Yi
    Jun, Nam-Soo
    Manigbas, Norvie L.
    Cho, Jun-Hyun
    Nam, Min-Hee
    Jeon, Jong-Seong
    Han, Chang-Deok
    Choi, Kyung-Jin
    Kim, Doh-Hoon
    Woo, Youngmin
    Koh, Hee-Jong
    Kang, Hang-Won
    Yi, Gihwan
    GENES & GENOMICS, 2009, 31 (04) : 277 - 282
  • [3] Molecular characterization and physico-chemical analysis of a new giant embryo mutant allele (get) in rice (Oryza sativa L.)
    Dong-Soo Park
    Soo-Kwon Park
    Bong-Chun Lee
    Song-Yi Song
    Nam-Soo Jun
    Norvie L. Manigbas
    Jun-Hyun Cho
    Min-Hee Nam
    Jong-Seong Jeon
    Chang-Deok Han
    Kyung-Jin Choi
    Doh-Hoon Kim
    Youngmin Woo
    Hee-Jong Koh
    Hang-Won Kang
    Gihwan Yi
    Genes & Genomics, 2009, 31 : 277 - 282
  • [4] Development of CoQ10-enriched rice from giant embryo lines
    Takahashi, Sakiko
    Ohtani, Toshikazu
    Iida, Shuichi
    Sunohara, Yoshihiro
    Matsushita, Kei
    Maeda, Hideo
    Tanetani, Yoshitaka
    Kawai, Kiyoshi
    Kawamukai, Makoto
    Kadowaki, Koh-ichi
    BREEDING SCIENCE, 2009, 59 (03) : 321 - 326
  • [5] Integrated transcriptomics and metabolomics analysis provide insight into the resistance response of rice against brown planthopper
    Shi, Shaojie
    Zha, Wenjun
    Yu, Xinying
    Wu, Yan
    Li, Sanhe
    Xu, Huashan
    Li, Peide
    Li, Changyan
    Liu, Kai
    Chen, Junxiao
    Yang, Guocai
    Chen, Zhijun
    Wu, Bian
    Wan, Bingliang
    Zhou, Lei
    You, Aiqing
    FRONTIERS IN PLANT SCIENCE, 2023, 14
  • [6] Creation and gene expression analysis of a giant embryo rice mutant with high GABA content
    Chen, Pingli
    Liu, Qing
    Sun, Bingrui
    Lv, Shuwei
    Jiang, Liqun
    Zhang, Jing
    Mao, Xingxue
    Yu, Hang
    Chen, Yangyang
    Chen, Wenfeng
    Fan, Zhilan
    Pan, Dajian
    Li, Chen
    MOLECULAR BREEDING, 2023, 43 (01)
  • [7] Creation and gene expression analysis of a giant embryo rice mutant with high GABA content
    Pingli Chen
    Qing Liu
    Bingrui Sun
    Shuwei Lv
    Liqun Jiang
    Jing Zhang
    Xingxue Mao
    Hang Yu
    Yangyang Chen
    Wenfeng Chen
    Zhilan Fan
    Dajian Pan
    Chen Li
    Molecular Breeding, 2023, 43
  • [8] Integrated Metabolomics and Transcriptomics Analysis Reveals New Insights into Triterpene Biosynthesis in Rosa rugosa
    Wei, Guo
    Xu, Yang
    Wang, Pengqing
    Hussain, Hammad
    Chen, Yudie
    Shi, Yuqing
    Zhu, Kaikai
    Bai, Mengjuan
    Xu, Yong
    Wang, Jianwen
    Feng, Liguo
    PLANTS-BASEL, 2024, 13 (12):
  • [9] Integrated Analysis of Metabolomics, Flavoromics, and Transcriptomics for Evaluating New Varieties of Amomum villosum Lour.
    Li, Zhenkai
    Luo, Xin
    Yao, Yanli
    Wang, Yukun
    Dai, Zhiheng
    Cheng, Tianle
    Huang, Xinzhi
    Bai, Mei
    He, Junjun
    Wu, Hong
    PLANTS-BASEL, 2024, 13 (17):
  • [10] Response of yield and quality of giant embryo rice to nitrogen application and analysis of lipid-lowering effect
    Hu, Mingming
    Chen, Guangyi
    Peng, Ligong
    Li, Congmei
    He, Xingmei
    Zhang, Qiuqiu
    Yang, Hong
    Liang, Chaode
    Kuang, Hudong
    Lan, Yan
    Li, Tian
    FRONTIERS IN PLANT SCIENCE, 2022, 13