Isolation and characterization of polymorphic microsatellite loci in the boll weevil/Anthonomus grandis Boheman (Coleoptera: Curculionidae)

被引：24

作者：

Kim, KS

Sappington, TW

机构：

[1] USDA ARS, Areawide Pest Management Res Unit, College Stn, TX 77845 USA

[2] Iowa State Univ, USDA, ARS, Corn Insects & Crop Genet Res Unit, Ames, IA 50011 USA

来源：

MOLECULAR ECOLOGY NOTES | 2004年 / 4卷 / 04期

关键词：

Anthonomus grandis; Boll weevil; microsatellites; molecular markers; population genetics;

D O I：

10.1111/j.1471-8286.2004.00765.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The boll weevil (Anthonomus grandis Boheman) is a major insect pest of cotton in North America. Dispersal activity poses a threat to ongoing eradication efforts in the US, but little is known about the frequency of long-distance migration. Nuclear molecular markers are needed to assess gene flow in relation to geographical distance. A biotin-enrichment strategy was employed to develop microsatellite markers for the boll weevil. Of 23 loci isolated, 14 were polymorphic with three to 10 alleles per locus. Twelve of the polymorphic loci showed Mendelian inheritance and are likely to be useful in population genetics studies.

引用

页码：701 / 703

页数：3

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