Porous Poly(vinyl alcohol) Composite Membranes for Immobilization of Glucose Oxidase

被引：3
|
作者：
Mojica, Ernesto Silva [1 ]
Lohrasbi, Mehdi [2 ]
Chuang, Steven S. C. [1 ]
机构：
[1] Univ Akron, Dept Polymer Sci, Akron, OH 44325 USA
[2] Univ Akron, Dept Chem & Biomol Engn, Akron, OH 44325 USA
来源：
TOPICS IN CATALYSIS | 2014年 / 57卷 / 17-20期
关键词：
Adsorption; Cross-linking; Enzyme; Glucose oxidase; Phase inversion; CONFORMATION;
D O I：
10.1007/s11244-014-0323-3
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
Particle loaded porous poly(vinyl alcohol) composite membranes were selected for immobilization of glucose oxidase (GOx) for their hydrophilicity and unique interactions with amino functional groups. GOx was immobilized on the membranes by adsorption at pH values between 3.5 and 7.1. The highest adsorption loading was observed at pH 7.1 and the highest catalytic activity was observed at pH 5.1. Infrared studies showed that the highest ratio of amide I to amide II at pH 5.1 is obtained for GOx immobilized on membranes loaded with amine-functionalized micro-particles, suggesting that the conformational changes of GOx on these membranes yield to higher catalytic activity than in other supports.
引用
收藏
页码：1490 / 1497
页数：8
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