Temperature dependence of collagen by Raman spectra

Raman spectra of collagen I at different temperature were obtained. There was no change at 1003 cm(-1) line, 1302 cm(-1) line moved to a higher wave number, but other lines moved to lower wave number when temperature increased. In addition, the authors observed the temperature dependence of Raman intensity and four denaturation points at 0, 40, 68 and 90 degreesC respectively. The points at 40 and 68 degreesC are in agreement with the experimental data by DSC and SHG. The point at 0 degreesC might be frozen transition; the point at 90 degreesC might be related to the damage of secondary structure. When heated to 150 degreesC, the Raman intensity of all bands decreased rapidly and many lines disappeared.