Amplification of Cherimoya (Annona cherimola Mill.) with Chloroplast-Specific Markers: Geographical Implications on Diversity and Dispersion Studies

被引：2

作者：

Larranaga, Nerea ^{[1
,2
]}

Fontecha, Gustavo ^{[3
]}

Albertazzi, Federico J. ^{[4
]}

Palmieri, Margarita ^{[5
]}

Hormaza, Jose, I ^{[1
]}

机构：

[1] UMA, Inst Hortofruticultura Subtrop & Mediterranea May, CSIC, Malaga 29750, Spain

[2] Univ Guadalajara, CONACYT, Dept Bot & Zool, Zapopan 45200, Mexico

[3] Univ Nacl Autonoma Honduras UNAH, Microbiol Res Inst, Tegucigalpa 11101, Honduras

[4] Univ Costa Rica, Ctr Invest Biol Celular & Mol CIBCM, San Jose 115012060, Costa Rica

[5] Univ Valle Guatemala UVG, Inst Invest UVG, Lab Protecc Vegetal, Ctr Estudios Agricolas & Alimentarios CEAA, Ciudad De Guatemala 01015, Guatemala

来源：

HORTICULTURAE | 2022年 / 8卷 / 09期

关键词：

Annona cherimola; specific marker; matK; trnL-F; haplotypes; DNA BARCODE; CONSERVATION;

D O I：

10.3390/horticulturae8090807

中图分类号：

S6 [园艺];

学科分类号：

0902 ;

摘要：

Previously developed Annona cherimola specific primers based on the plant barcode gene matK were tested in 546 cherimoya accessions. Of those, 296 belong to an ex situ world reference germplasm collection maintained at the IHSM La Mayora-CSIC-UMA in Malaga (Spain) and 250 to cherimoya samples collected in situ in three Central American countries (Honduras, Guatemala and Costa Rica). Results showed the existence of two different haplotypes in the samples analyzed with geographical association. A new marker that amplified all the samples was designed based on the trnL-F locus using DNA information from seven species of the Annona genus. The information generated will be useful to analyze and conserve the extant genetic diversity present in situ and ex situ and to understand de dispersion of the crop from its center of origin in Central America.

引用

页数：10

共 5 条

[1] Characterization and cross-species amplification of microsatellite markers in cherimoya (Annona cherimola Mill., Annonaceae)
Escribano, P
Viruel, MA
Hormaza, JI
MOLECULAR ECOLOGY NOTES, 2004, 4 (04): : 746 - 748
[2] Value chains of cherimoya (Annona cherimola Mill.) in a centre of diversity and its on-farm conservation implications
Vanhove, Wouter
Van Damme, Patrick
TROPICAL CONSERVATION SCIENCE, 2013, 6 (02): : 158 - 180
[3] A Mesoamerican origin of cherimoya (Annona cherimola Mill.): Implications for the conservation of plant genetic resources
Larranaga, N.
Albertazzi, F. J.
Fontecha, G.
Palmieri, M.
Rainer, H.
van Zonneveld, M.
Hormaza, J. I.
MOLECULAR ECOLOGY, 2017, 26 (16) : 4116 - 4130
[4] Development of 52 new polymorphic SSR markers from cherimoya (Annona cherimola Mill.):: transferability to related taxa and selection of a reduced set for DNA fingerprinting and diversity studies
Escribano, P.
Viruel, M. A.
Hormaza, J. I.
MOLECULAR ECOLOGY RESOURCES, 2008, 8 (02): : 317 - 321
[5] Mapping Genetic Diversity of Cherimoya (Annona cherimola Mill.): Application of Spatial Analysis for Conservation and Use of Plant Genetic Resources
van Zonneveld, Maarten
Scheldeman, Xavier
Escribano, Pilar
Viruel, Maria A.
Van Damme, Patrick
Garcia, Willman
Tapia, Cesar
Romero, Jose
Siguenas, Manuel
Hormaza, Jose I.
PLOS ONE, 2012, 7 (01):

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