The clinical need for clustered AChR cell-based assay testing of seronegative MG

被引：9

作者：

Masi, Gianvito ^{[1
,2
]}

Li, Yingkai ^{[3
]}

Karatz, Tabitha ^{[3
]}

Pham, Minh C. ^{[2
]}

Oxendine, Seneca R. ^{[1
,2
]}

Nowak, Richard J. ^{[1
]}

Guptill, Jeffrey T. ^{[3
,4
]}

'Connor, Kevin C. O. ^{[1
,2
,5
]}

机构：

[1] Yale Sch Med, Dept Neurol, New Haven, CT 06511 USA

[2] Yale Sch Med, Dept Immunobiol, New Haven, CT 06511 USA

[3] Duke Univ Med Ctr, Dept Neurol, Neuromuscular Div, Durham, NC 27710 USA

[4] Duke Clin Res Inst, Durham, NC 27710 USA

[5] Yale Sch Med, 300 George St Room 353J, New Haven, CT 06511 USA

来源：

JOURNAL OF NEUROIMMUNOLOGY | 2022年 / 367卷

关键词：

Myasthenia gravis; Seronegative; Cell-based assay; Autoantibodies; MG myasthenia gravis; MuSK muscle-specific tyrosine kinase; RIA radioimmunoassay; SN seronegative; AUTOANTIBODIES; ANTIBODIES;

D O I：

10.1016/j.jneuroim.2022.577850

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Trial eligibility in myasthenia gravis (MG) remains largely dependent on a positive autoantibody serostatus. This significantly hinders seronegative MG (SNMG) patients from receiving potentially beneficial new treatments. In a subset of SNMG patients, acetylcholine receptor (AChR) autoantibodies are detectable by a clustered AChR cell based assay (CBA). Of 99 SNMG patients from two academic U.S. centers, 18 (18.2%) tested positive by this assay. Autoantibody positivity was further validated in 17/18 patients. In a complementary experiment, circulating AChR-specific B cells were identified in a CBA-positive SNMG patient. These findings corroborate the clinical need for clustered AChR CBA testing when evaluating SNMG patients.

引用

页数：4

共 50 条

[1] THE UNMET CLINICAL NEED FOR CLUSTERED ACETYLCHOLINE RECEPTOR CELL-BASED ASSAY TESTING IN PATIENTS WITH SERONEGATIVE MYASTHENIA GRAVIS
Masi, Gianvito
Li, Yingkai
Karatz, Tabitha
Pham, Minh C.
Oxendine, Seneca
Nowak, Richard J.
Guptill, Jeffrey T.
O'Connor, Kevin C.
[J]. MUSCLE & NERVE, 2022, 65 : S17 - S17
[2] A Stable Cell Line Expressing Clustered AChR: A Novel Cell-Based Assay for Anti-AChR Antibody Detection in Myasthenia Gravis
Cai, Yu
Han, Lu
Zhu, Desheng
Peng, Jing
Li, Jianping
Ding, Jie
Luo, Jiaying
Hong, Ronghua
Wang, Kan
Wan, Wenbin
Xie, Chong
Zhou, Xiajun
Zhang, Ying
Hao, Yong
Guan, Yangtai
[J]. FRONTIERS IN IMMUNOLOGY, 2021, 12
[3] IgG-specific cell-based assay detects potentially pathogenic MuSK-Abs in seronegative MG
Huda, Saif
Waters, Patrick
Woodhall, Mark
Leite, Maria Isabel
Jacobson, Leslie
De Rosa, Anna
Maestri, Michelangelo
Ricciardi, Roberta
Heckmann, Jeannine M.
Maniaol, Angelina
Evoli, Amelia
Cossins, Judy
Hilton-Jones, David
Vincent, Angela
[J]. NEUROLOGY-NEUROIMMUNOLOGY & NEUROINFLAMMATION, 2017, 4 (04):
[4] Clinical value of cell-based assays in the characterisation of seronegative myasthenia gravis
Damato, Valentina
Spagni, Gregorio
Monte, Gabriele
Woodhall, Mark
Jacobson, Leslie
Falso, Silvia
Smith, Thomas
Iorio, Raffaele
Waters, Patrick
Irani, Sarosh R.
Vincent, Angela
Evoli, Amelia
[J]. JOURNAL OF NEUROLOGY NEUROSURGERY AND PSYCHIATRY, 2022, 93 (09): : 995 - 1000
[5] Comparison of Fixed and Live Cell-Based Assay for the Detection of AChR and MuSK Antibodies in Myasthenia Gravis
Spagni, Gregorio
Gastaldi, Matteo
Businaro, Pietro
Chemkhi, Zeineb
Carrozza, Cinzia
Mascagna, Giovanni
Falso, Silvia
Scaranzin, Silvia
Franciotta, Diego
Evoli, Amelia
Damato, Valentina
[J]. NEUROLOGY-NEUROIMMUNOLOGY & NEUROINFLAMMATION, 2023, 10 (01):
[6] Fixed cell-based assay vs live cell-based assay for MOGAD
Carta, S.
Pique, J.
Goncalvez, D.
Deschamps, R.
Mathey, G.
Cohen, M.
Chaumont, H.
Wang, A.
Zepir, H.
Tourniaire, P.
Fabien, N.
Marignier, R.
[J]. EUROPEAN JOURNAL OF NEUROLOGY, 2023, 30 : 385 - 386
[7] Validation of a Live Cell-Based Assay For Antibodies to Clustered Acetlycholine Receptor Antibodies in Myasthenia Gravis
Cruz, Anna
Frykman, Hans
[J]. NEUROLOGY, 2020, 94 (15)
[8] The development of a stem cell-based assay for quality control testing in IVF
Adjirackor, N.
Beckitt, T.
Fowler, K.
Harvey, S.
[J]. HUMAN REPRODUCTION, 2020, 35 : 1506 - 1507
[9] LIVE CELL-BASED ASSAY FOR ANTIBODIEST CLUSTERED ACETYLCHOLINE RECEPTOR IN MYASTHENIA GRAVIS, CROSS VALIDATION, INTER-ASSAY STABILITY AND UTILITY IN A PEDIATRIC COHORT SUSPECTED FOR MG
Cruz, A.
Frykman, H.
[J]. MUSCLE & NERVE, 2020, 62 : S123 - S123
[10] Pathogenic antibodies in neuromyelitis optica: Increased sensitivity of cell-based assay with clustered aquaporin-4
Patrick, Waters
Angela, Vincent
Isabel, Leite M.
[J]. JOURNAL OF NEUROIMMUNOLOGY, 2010, 228 (1-2) : 160 - 160

← 1 2 3 4 5 →