CONFOCAL MICROSCOPIC ANALYSIS OF THE MICROFILAMENT CONFIGURATIONS FROM HUMAN VITRIFICATION-THAWED OOCYTES MATURED IN VITRO

被引：0
|
作者：
Ci, Qianqian [1 ,2 ]
Li, Mei [1 ]
Zhang, Yingchun [2 ]
Ma, Shuiying [1 ]
Gao, Qin [1 ]
Shi, Yuhua [1 ]
机构：
[1] Shandong Univ, Shandong Prov Hosp, Ctr Reprod Med, Jinan 250100, Peoples R China
[2] Shandong Univ, Jinan Cent Hosp, Ctr Reprod Med, Jinan 250100, Peoples R China
来源：
CRYOLETTERS | 2014年 / 35卷 / 06期
基金：
中国国家自然科学基金;
关键词：
oocyte; in vitro maturation; vitrification; microfilament; confocal microscopy; CRYOPRESERVATION; SPINDLE; CYTOSKELETAL;
D O I：
暂无
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
BACKGROUND: The alteration in microfilaments of human oocytes by vitrification has not been understood well. OBJECTIVE: To evaluate the effect of vitrification on the microfilaments of human in vitro matured oocytes and the time needed for the repair. MATERIALS AND METHODS: Human in vitro matured oocytes were divided into the control group (Group 1) and the vitrified group. The vitrified oocytes were further divided into four sub-groups that were cultured after thawing for 1h, 2h, 3h and 4h, respectively (Groups 2, 3, 4 and 5). RESULTS: The survival rate of oocytes was 87.4% after vitrification and thawing. The percentage of oocytes with a normal configuration of microfilaments in Group 2 and Group 3 was significantly lower than that of the fresh control group (Group 1), whereas the percentage of oocytes with normal microfilament configuration in Group 4 and Group 5 was comparable to the control group. CONCLUSION: Vitrification alters microfilament structure of oocytes, which takes at least 3h after thawing for the repair and recovery.
引用
收藏
页码：544 / 548
页数：5
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