This study was performed to compare the effects of brewing method (decoction and infusion), time (1-5 min), and also origin of coffee beans on antioxidant activity using DPPH, ABTS, FRAP methods, caffeine content and phenolic compounds by HPLC. Principal component analysis was used to determine the effective treatments on bioactive properties. Coffee brews prepared with decoction method contained higher contents of total phenolic, total flavonoid, and total tannin as compared to the infusion method. On the other hand, antioxidant activity of coffee brews by DPPH and ABTS radical methods showed an opposite trend. Similar to antioxidant activity, infusion method was better to obtain higher amounts of catechin, caffeic acid and quercetin in coffee brews. The brewing method did not cause a significant difference in caffeine and rutin contents. Brewing time had major effects on several bioactive compounds, and their amounts showed an increase after extending the time of brewing.
机构:
Univ Fed Rio de Janeiro, Dept Bioquim, Inst Quim, Lab Bioquim Nutr & Alimentos, BR-21941 Rio De Janeiro, BrazilUniv Fed Rio de Janeiro, Dept Bioquim, Inst Quim, Lab Bioquim Nutr & Alimentos, BR-21941 Rio De Janeiro, Brazil
Perrone, Daniel
Farah, Adriana
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Univ Fed Rio de Janeiro, Dept Bioquim, Inst Quim, Lab Bioquim Nutr & Alimentos, BR-21941 Rio De Janeiro, Brazil
Univ Fed Rio de Janeiro, Nucleo Pesquisa Cafe Prof Luiz Carlos Trugo, Lab Quim & Bioatividade Alimentos, Inst Nutr, BR-21941 Rio De Janeiro, BrazilUniv Fed Rio de Janeiro, Dept Bioquim, Inst Quim, Lab Bioquim Nutr & Alimentos, BR-21941 Rio De Janeiro, Brazil
Farah, Adriana
Donangelo, Carmen M.
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Univ Fed Rio de Janeiro, Dept Bioquim, Inst Quim, Lab Bioquim Nutr & Alimentos, BR-21941 Rio De Janeiro, Brazil
Univ Republica, Escuela Nutr & Dietet, Montevideo, UruguayUniv Fed Rio de Janeiro, Dept Bioquim, Inst Quim, Lab Bioquim Nutr & Alimentos, BR-21941 Rio De Janeiro, Brazil