Eighty-six species of stoneflies are reported from Colorado, including a new species of Capnia. Three new state records are reported, Bolshecapnia milami (Nebeker and Gaufin), Capnura fibula (Claassen), and lsoperla marlynia (Needham and Claassen). Ninety percent of all Colorado stoneflies are typical western North American species, with seven species, Paracapnia angulata Hanson, Taeniopteryx burksi Ricker and Ross, T. parvula Banks, I.marlynia, Acroneuria abnormis (Newman), Perlesta decipiens (Walsh), and Isoperla bilineata (Say) having eastern affinities. Arcynopteryx compacta (McLachlan) is considered circumpolar species.