Development of novel microsatellite markers for the spider genus Loxosceles (Sicariidae) using next-generation sequencing

被引：5
|
作者：
Planas, Enric [1 ]
Bernaus, Laia
Ribera, Caries
机构：
[1] Univ Barcelona, Fac Biol, Inst Recerca Biodivers IRBio, E-08028 Barcelona, Spain
来源：
JOURNAL OF ARACHNOLOGY | 2014年 / 42卷 / 03期
关键词：
454; nuclear markers; cross-amplification; Mediterranean; Canary Islands; CANARY-ISLANDS; PATTERNS;
D O I：
10.1636/B13-88.1
中图分类号：
Q96 [昆虫学];
学科分类号：
摘要：
We report the step-by-step process of developing de novo microsatellite (SSR) loci in two Loxosceles spider species. We used reads obtained with next-generation sequencing (Roche 454) to select hundreds of potentially-amplifiable SSRs. After testing amplification and cross-amplification, we characterized 18 SSRs, 11 of which were polymorphic in Loxosceles rufescens (Dufour 1820) and seven of which were polymorphic in L. sp. Fuerteventura - Lanzarote. This method is a relatively fast and economic procedure for the development of fast-evolving nuclear markers in spiders.
引用
收藏
页码：315 / 317
页数：3
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