The effect of modification of nucleotide-37 on the interaction of aminoacyl-tRNA with the a site of the 70S ribosome

被引：0
|
作者：
Soboleva, NG [1 ]
Makhno, VI [1 ]
Konevega, AL [1 ]
Semenkov, YP [1 ]
Katunin, VI [1 ]
机构：
[1] Russian Acad Sci, Konstantinov Inst Nucl Phys, Gatchina 188300, Leningrad Regio, Russia
来源：
MOLECULAR BIOLOGY | 2003年 / 37卷 / 01期
关键词：
tRNA(Phe); 70S ribosome; A site; modified nucleotide; Y-base;
D O I：
10.1023/A:1022345115927
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
To estimate the effect of modified nucleotide 37, the interaction of two yeast aminoacyl-tRNAs (Phe-tRNA (Phe)(+Y) and Phe-tRNA Pbe(-Y)(Phe)) with the A site of complex [70S . poly(U) . deacylated tRNA(Phe) in the P site] was assayed at 0-20degreesC. As comparisons with native Phe-tRNA(+Y)(Phe) showed, removal of the Y base decreased the association constant of Phe-tRNA(-Y)(Phe) and the complex by an order of magnitude at every temperature tested, and increased the enthalpy of their interaction by 23 kJ/mol. When the Y base was present in the anticodon loop of deacylated tRNAPhe bound to the P site of the 70S ribosome, twice higher affinity for the A site was observed for Phe-tRNA(-Y)(Phe) but not for the Phe-tRNA(+Y)(Phe) Thus, the modified nucleotide 3' of the Phe-tRNA(Phe) anticodon stabilized the codon-anticodon interaction both in the A and P sites of the 70S ribosome.
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页码：110 / 115
页数：6
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