Biocompatible electrospun papain/chitosan NP/PVA nanofibrous scaffolds

The development and depiction of papain-incorporated chitosan/poly(vinyl alcohol) (PVA) nanofibres synthesized through electrospinning were studied for controlled bioengineering applications. Papain is a natural proteolytic enzyme, capable of breaking down larger protein particles into smaller peptides and helps in wound healing. Both chitosan and PVA possess outstanding properties like biocompatibility, biodegradability and non-toxic in nature. The morphological surface of the synthesized chitosan nanoparticles and electrospun nanofibres were analysed using scanning electron microscopy and the functional groups using Fourier transform infrared analyses. The antibacterial activity studied against Gram-positive and Gram-negative bacteria predicted the acceptability of the prepared papain/chitosan/PVA nanofibre to find its application in the process of wound healing.