The family Tischeriidae is reported from the Galapagos Islands for the first time. The two species found on the archipelago (Astrotischeria scalesiaella B. Landry, sp. n. and A. alcedoensis B. Landry, sp. n.) are described and illustrated. Both species are presumed to be endemic and their larvae are leaf miners on Scalesia species, a group of endemic Asteraceae.