Quality and Fertilizing Ability In Vivo of Sex-Sorted Stallion Spermatozoa

被引：13

作者：

Mari, G. ^{[1
]}

Rizzato, G. ^{[1
]}

Merlo, B. ^{[1
]}

Iacono, E. ^{[1
]}

Bucci, D. ^{[2
]}

Seren, E. ^{[2
]}

Tamanini, C. ^{[2
]}

Galeati, G. ^{[2
]}

Spinaci, M. ^{[2
]}

机构：

[1] Univ Bologna, Vet Clin Dept, I-40064 Bologna, Italy

[2] Univ Bologna, DIMORFIPA, I-40064 Bologna, Italy

来源：

REPRODUCTION IN DOMESTIC ANIMALS | 2010年 / 45卷 / 02期

关键词：

LOW NUMBERS; MAMMALIAN SPERM; HYSTEROSCOPIC INSEMINATION; INTRAUTERINE INSEMINATION; MEMBRANE INTEGRITY; CHROMOSOME-BEARING; BOAR SPERMATOZOA; SEMINAL PLASMA; X-CHROMOSOME; Y-SPERM;

D O I：

10.1111/j.1439-0531.2008.01314.x

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

Contents Little information is available on the quality of stallion spermatozoa after sex sorting. The objectives of the present study were to assess the quality of sex-sorted stallion spermatozoa and determine its fertilizing ability after hysteroscopic low dose insemination. Ejaculates from four stallions were collected and sorted by a MoFlo SX (R) flow cytometer/sperm sorter. Before and after sorting, spermatozoa were evaluated for motility by Computer Assisted Sperm Analysis, viability (SYBR 14-propidium iodide), mitochondrial function (JC-1) and acrosomal status (fluorescein isothiocyanate Pisum sativum agglutinin conjugated). A fertility trial was carried out on four mares (seven oestrous cycles) by hysteroscopic insemination, depositing 5 x 106 X-bearing spermatozoa. Sex sorting resulted in a significant decrease (p < 0.001) in all motility characteristics. Sperm viability and percentage of spermatozoa with functional mitochondria were not affected by the sorting process, while the percentage of reacted spermatozoa was higher (p < 0.01) for non-sorted than sorted spermatozoa. Pregnancy rate was 28.6% (2/7) after low dose hysteroscopic insemination. Only one pregnancy was carried to term with the birth of a healthy filly. In conclusion, despite the reduction in sperm motility, sex sorting did not impair stallion sperm viability and mitochondrial activity immediately post-thaw; moreover, the sexed spermatozoa retained the ability to fertilize in vivo.

引用

页码：331 / 335

页数：5

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