Amorphous Calcium Carbonate Precipitation by Cellular Biomineralization in Mantle Cell Cultures of Pinctada fucata

被引:42
|
作者
Xiang, Liang [1 ,2 ]
Kong, Wei [1 ]
Su, Jingtan [1 ]
Liang, Jian [1 ]
Zhang, Guiyou [1 ]
Xie, Liping [1 ,3 ]
Zhang, Rongqing [1 ,3 ]
机构
[1] Tsinghua Univ, Sch Life Sci, Inst Marine Biotechnol, Beijing 100084, Peoples R China
[2] Chinese Acad Sci, Shenzhen Inst Adv Technol, Inst Biomed & Biotechnol, Lab Reprod Hlth, Shenzhen, Peoples R China
[3] Tsinghua Univ, Prot Sci Lab, Minist Educ, Beijing 100084, Peoples R China
来源
PLOS ONE | 2014年 / 9卷 / 11期
基金
中国国家自然科学基金; 高等学校博士学科点专项科研基金; 国家高技术研究发展计划(863计划);
关键词
MATRIX PROTEIN SECRETION; PEARL OYSTER; PRECURSOR PHASE; SHELL FORMATION; MOLLUSK; TISSUE; NACRE; ARAGONITE; ABALONE; LAYER;
D O I
10.1371/journal.pone.0113150
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
The growth of molluscan shell crystals is generally thought to be initiated from the extrapallial fluid by matrix proteins, however, the cellular mechanisms of shell formation pathway remain unknown. Here, we first report amorphous calcium carbonate (ACC) precipitation by cellular biomineralization in primary mantle cell cultures of Pinctada fucata. Through real-time PCR and western blot analyses, we demonstrate that mantle cells retain the ability to synthesize and secrete ACCBP, Pif80 and nacrein in vitro. In addition, the cells also maintained high levels of alkaline phosphatase and carbonic anhydrase activity, enzymes responsible for shell formation. On the basis of polarized light microscopy and scanning electron microscopy, we observed intracellular crystals production by mantle cells in vitro. Fourier transform infrared spectroscopy and X-ray diffraction analyses revealed the crystals to be ACC, and de novo biomineralization was confirmed by following the incorporation of Sr into calcium carbonate. Our results demonstrate the ability of mantle cells to perform fundamental biomineralization processes via amorphous calcium carbonate, and these cells may be directly involved in pearl oyster shell formation.
引用
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页数:8
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