ATP-dependent Lon protease from maize mitochondria -: comparison with the other Lon proteases

被引：0
|
作者：
Mertová, J
Almásiová, M
Perecko, D
Bilka, F
Benesová, M
Bezáková, L
Psenák, M
Kutejová, E
机构：
[1] Slovak Acad Sci, Inst Mol Biol, SK-84551 Bratislava, Slovakia
[2] Comenius Univ, Fac Pharm, Dept Cell & Mol Biol Drugs, SK-83232 Bratislava, Slovakia
来源：
BIOLOGIA | 2002年 / 57卷 / 06期
关键词：
ATP-dependent protease; Lon protease; mitochondria; maize; plants;
D O I：
暂无
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
ATP-dependent protease has been identified in the mitochondrial matrix of Zea mays L. Sachara. Enzyme has been purified 139-fold. Enzyme digests [(14)C]-methylated beta-casein yielding TCA-soluble peptides and needs ATP and Mg(2+) ions for its activity and stability. Inhibitory profile corresponds to those of ATP-dependent serine proteases of Lon (La) family. Optimal pH is 8.5-9.0 and optimal temperature 40-45degreesC, suggesting the heat shock character of the enzyme. Basic enzymatic characteristics of maize mitochondrial protease are similar to those of corresponding Lon protease from rat liver and yeast mitochondria and of E. coli protease La. ATP-dependent protease from maize mitochondria seems to be a heptamer with considerably less stability than other mitochondrial Lon proteases.
引用
收藏
页码：739 / 745
页数：7
相关论文
共 50 条
  • [1] Multitasking in the mitochondrion by the ATP-dependent Lon protease
    Venkatesh, Sundararajan
    Lee, Jae
    Singh, Kamalendra
    Lee, Irene
    Suzuki, Carolyn K.
    BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH, 2012, 1823 (01): : 56 - 66
  • [2] Biological roles of the Lon ATP-dependent protease
    Tsilibaris, Virginie
    Maenhaut-Michel, Genevieve
    Van Melderen, Laurence
    RESEARCH IN MICROBIOLOGY, 2006, 157 (08) : 701 - 713
  • [3] Mitochondrial ATP-Dependent Lon Protease and Cancer
    Lan, L.
    Huang, K.
    Liu, Y.
    Lu, B.
    PANCREAS, 2017, 46 (07) : 956 - 957
  • [4] Structural studies of bacterial Lon ATP-dependent proteases
    Duman, Ramona
    Lowe, Jan
    ACTA CRYSTALLOGRAPHICA A-FOUNDATION AND ADVANCES, 2011, 67 : C354 - C354
  • [5] Classification of ATP-dependent proteases Lon and comparison of the active sites of their proteolytic domains
    Rotanova, TV
    Melnikov, EE
    Khalatova, AG
    Makhovskaya, OV
    Botos, I
    Wlodawer, A
    Gustchina, A
    EUROPEAN JOURNAL OF BIOCHEMISTRY, 2004, 271 (23-24): : 4865 - 4871
  • [6] Slicing a protease: Structural features of the ATP-dependent Lon proteases gleaned from investigations of isolated domains
    Rotanova, Tatyana V.
    Botos, Istvan
    Melnikov, Edward E.
    Rasulova, Fatima
    Gustchina, Alla
    Maurizi, Michael R.
    Wlodawer, Alexander
    PROTEIN SCIENCE, 2006, 15 (08) : 1815 - 1828
  • [7] ATP-DEPENDENT PROTEASE LA (LON) FROM ESCHERICHIA-COLI
    GOLDBERG, AL
    MOERSCHELL, RP
    CHUNG, CH
    MAURIZI, MR
    PROTEOLYTIC ENZYMES: SERINE AND CYSTEINE PEPTIDASES, 1994, 244 : 350 - 375
  • [8] Cloning and characterization of the murine mitochondrial ATP-dependent Lon protease
    Liu, T
    Lu, B
    Crosby, JA
    Lee, I
    Suzuki, CK
    MOLECULAR BIOLOGY OF THE CELL, 2001, 12 : 384A - 384A
  • [9] Investigating nucleotide interaction in the ATP-dependent protease Lon.
    Thomas-Wohlever, J
    Behshad, R
    Burke, M
    Lee, I
    BIOCHEMISTRY, 2003, 42 (28) : 8603 - 8603
  • [10] ATP-Dependent Lon Proteases in the Cellular Protein Quality Control System
    A. M. Kudzhaev
    A. G. Andrianova
    A. E. Gustchina
    I. V. Smirnov
    T. V. Rotanova
    Russian Journal of Bioorganic Chemistry, 2022, 48 : 678 - 709
12345