Development of a Near-infrared Laser-induced Surface Deformation Microscope and Its Application to the Dynamic Viscoelastic Measurements of Single Living Plant Cell Surfaces

被引：1

作者：

Morisaku, Toshinori ^{[1
]}

Onuki, Hitomi ^{[2
]}

Hashimoto, Kenji ^{[3
]}

Kuchitsu, Kazuyuki ^{[3
,4
]}

Yui, Hiroharu ^{[1
,2
]}

机构：

[1] Tokyo Univ Sci, Res Inst Sci & Technol, Water Frontier Sci & Technol Res Ctr, Shinjuku Ku, 1-3 Kagurazaka, Tokyo 1628601, Japan

[2] Tokyo Univ Sci, Fac Sci, Dept Chem, Shinjuku Ku, 1-3 Kagurazaka, Tokyo 1628601, Japan

[3] Tokyo Univ Sci, Fac Sci & Technol, Dept Appl Biol Sci, 2641 Yamazaki, Noda, Chiba 2788510, Japan

[4] Tokyo Univ Sci, Res Inst Sci & Technol, Imaging Frontier Ctr, 2641 Yamazaki, Noda, Chiba 2788510, Japan

来源：

ANALYTICAL SCIENCES | 2019年 / 35卷 / 11期

基金：

日本学术振兴会;

关键词：

LISD microscope; near-infrared laser; dynamic viscoelasticity; plant cell; FORCE; WALLS;

D O I：

10.2116/analsci.19P227

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A near-infrared laser-induced surface deformation (NIR-LISD) microscope is developed and is applied to the dynamic viscoelastic measurements of the surface of a living plant cell. In the microscope, the deformation of the surface is induced by an NIR laser beam, and then the change in intensity of the probe beam reflected from the surface reflects its viscoelasticity. The application of the NIR laser beam has a great advantage for the prevention of damage to the plant cell compared to the irradiation of a visible laser beam in LISD measurements. The NIR-LISD microscope allows for discriminating the differences in power spectra between the subapical and lateral regions of single rhizoids. It is a useful method for the dynamic viscoelastic measurements of cells, such as plant cells, that are damaged due to the strong absorption of ultraviolet or visible light.

引用

页码：1203 / 1207

页数：5

共 7 条

[1] Development of a Near-infrared Laser-induced Surface Deformation Microscope and Its Application to the Dynamic Viscoelastic Measurements of Single Living Plant Cell Surfaces
Toshinori Morisaku
Hitomi Onuki
Kenji Hashimoto
Kazuyuki Kuchitsu
Hiroharu Yui
Analytical Sciences, 2019, 35 : 1203 - 1207
[2] Laser-induced surface deformation microscope for the study of the dynamic viscoelasticity of plasma membrane in a living cell
Morisaku, Toshinori
Yui, Hiroharu
ANALYST, 2018, 143 (10) : 2397 - 2404
[3] Discrimination between Normal and Cancerous Cells from Dynamic Viscoelastic Properties with a Laser-induced Surface Deformation Microscope
Morisaku, Toshinori
Ishihara, Masashi
Yui, Hiroharu
ANALYTICAL SCIENCES, 2018, 34 (08) : 979 - 982
[4] Discrimination between Normal and Cancerous Cells from Dynamic Viscoelastic Properties with a Laser-induced Surface Deformation Microscope
Toshinori Morisaku
Masashi Ishihara
Hiroliani Yui
Analytical Sciences, 2018, 34 : 979 - 982
[5] Development of near-infrared laser-induced capillary wave method to measure viscosity and surface tension
Takiguchi, Hiroki
Nagasaka, Yuji
Nihon Kikai Gakkai Ronbunshu, B Hen/Transactions of the Japan Society of Mechanical Engineers, Part B, 2013, 79 (800): : 690 - 700
[6] Development of near-infrared 35 fs laser microscope and its application to the detection of three- and four-photon fluorescence of organic microcrystals
Matsuda, H
Fujimoto, Y
Ito, S
Nagasawa, Y
Miyasaka, H
Asahi, T
Masuhara, H
JOURNAL OF PHYSICAL CHEMISTRY B, 2006, 110 (03): : 1091 - 1094
[7] Laser-Induced Intracellular Delivery: Exploiting Gold-Coated Spiky Polymeric Nanoparticles and Gold Nanorods under Near-Infrared Pulses for Single-Cell Nano-Photon-Poration
Kumar, Ashish
Nahak, Bishal Kumar
Gupta, Pallavi
Santra, Tuhin Subhra
Tseng, Fan-Gang
MICROMACHINES, 2024, 15 (02)

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