Isolation and characterization of microsatellite markers in the South African abalone (Haliotis midae)

被引：18
|
作者：
Bester, AE [1 ]
Slabbert, R [1 ]
D'Amato, ME [1 ]
机构：
[1] Univ Stellenbosch, Dept Genet, ZA-7602 Stellenbosch, South Africa
来源：
MOLECULAR ECOLOGY NOTES | 2004年 / 4卷 / 04期
关键词：
abalone; Haliotis; microsatellites;
D O I：
10.1111/j.1471-8286.2004.00755.x
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We report the isolation and characterization of 11 polymorphic microsatellite loci in the South African abalone Haliotis midae. These loci showed a range of five to 21 alleles per locus and observed heterozygosities ranging from 0.14 to 0.93 in a wild population of 32 individuals. All loci except four conformed to Hardy-Weinberg expectations and did not show linkage disequilibrium. The polymorphism exhibited at these loci indicate that they would be useful in determining levels of genetic variability in natural and commercial Haliotis midae populations as well as in parentage and Quantitative Trait Loci (QTL) analysis in hatchery reared abalone.
引用
收藏
页码：618 / 619
页数：2
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