Observing DNA in live cells

被引：14
|
作者：
Weiss, Lucien E. [1 ]
Naor, Tal [1 ]
Shechtman, Yoav [1 ]
机构：
[1] Technion Israel Inst Technol, Dept Biomed Engn, IL-32000 Haifa, Israel
来源：
BIOCHEMICAL SOCIETY TRANSACTIONS | 2018年 / 46卷
关键词：
IN-SITU; LOCALIZATION MICROSCOPY; CHROMOSOME DYNAMICS; CHROMATIN DYNAMICS; DIFFRACTION-LIMIT; BREAK REPAIR; VISUALIZATION; RNA; RESOLUTION; SEQUENCES;
D O I：
10.1042/BST20170301
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The structural organization and dynamics of DNA are known to be of paramount importance in countless cellular processes, but capturing these events poses a unique challenge. Fluorescence microscopy is well suited for these live-cell investigations, but requires attaching fluorescent labels to the species under investigation. Over the past several decades, a suite of techniques have been developed for labeling and imaging DNA, each with various advantages and drawbacks. Here, we provide an overview of the labeling and imaging tools currently available for visualizing DNA in live cells, and discuss their suitability for various applications.
引用
收藏
页码：729 / 740
页数：12
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