Understanding the mechanisms of transmission of plant viruses is an important part of devising effective and sustainable strategies to protect crop plants against plant virus diseases. There are many difficulties associated with the study of virus transmission by nematodes, particularly as these vector organisms live below ground in the soil feeding on plant roots and cannot be maintained in pure culture. Nevertheless, with recent advances in molecular cloning techniques many details of the transmission process have begun to be revealed, especially with regard to the virus proteins that are required for successful transmission.