Natural and mutant strains of A. chroococcum were isolated from Indian soils. Their ability to dissolve phosphate and their phytohormone production were tested under in vitro conditions. In addition the effect of bacterial inoculation of Azotobacter on N, P, K uptake by three P responsive wheat,genotypes (Triticum aestivum L.) under greenhouse conditions at five nutrient levels Control, 90 kg N ha(-1), 90 kg N + 26 kg P ha(-1), 120 kg N ha(-1) and 120 kg N + 26 kg P ha(-1)) was studied. In vitro phosphate Solubilization and growth hormone production by mutant strains was more than by the soil isolates. Inoculation of wheat varieties with the soil isolates and mutant strains of A. chroococcum showed greater NPK uptakes as compared with parent soil isolates. Mutant strains M15 and M37 were proved to be the most effective for all three wheat varieties with regard to NPK uptake as well as root biomass production under greenhouse conditions.