Nocardioides jejuensis sp. nov., isolated from soil

A Gram-stain positive, asporogenous, aerobic, white -coloured bacterium, designated 18JY15-6(T), was isolated from soil from Jeju Island, Korea. Pairwise analysis of the 16S rRNA gene sequence of strain 18JY15-6(T) indicated high similarity to Nocardioides phosphati DSM 104026(T) (97.4%), Marmoricola terrae KACC 17308(T) (96.7%) and Nocardioides jensenii KCTC 0074BP(T) (96.6%). Phylogenetic analysis revealed that strain 18JY15-6(T) formed a distinct lineage within the family Nocardioidaceae and is closely related to members of the genus Nocardioides. Genome sequencing of strain 18JY15-6(T) revealed 3221 total genes, including 3162 protein coding genes, 59 RNA and 31 pseudogenes. Growth was observed at 18-37 degrees C (optimal 30 degrees C) in R2A medium at pH 7.0. The major cellular fatty acids of strain 18JY15-6(T) were identified as C-16:0, C-18:1 omega 9c, C-18:0 10-methyl, tuberculostearic and C-17:0. The fatty acid profile of strain 18JY15-6(T) was more dissimilar when compared with M. terrae. The only respiratory quinone present was found to be MK-8(H-4). The major polar lipids are diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and phosphatidylcholine. The results of phylogenetic, biochemical and chemotaxonomic characterisation allow the differentiation of strain 18JY15-6(T) from N. phosphati WYH11-7(T), M. terrae JOS5-1(T) and N. jensenii NBRC 14755(T) which supports the conclusion that this strain represents a novel species of the genus Nocardioides, for which we propose the name Nocardioides jejuensis sp. nov. The type strain of Nocardioides jejuensis is 18JY15-6(T) (= KCTC 49105(T) = JCM 33182(T)).