Modified poly(caprolactone trifumarate) with embedded gelatin microparticles as a functional scaffold for bone tissue engineering

被引:6
|
作者
Al-Namnam, Nisreen Mohammed Nagi [1 ]
Kim, Kah Hwi [2 ]
Chai, Wen Lin [3 ]
Ha, Kien Oon [1 ]
Siar, Chong Huat [1 ]
Ngeow, Wei Cheong [1 ]
机构
[1] Univ Malaya, Fac Dent, Dept Oromaxillofacial Surg & Med Sci, Kuala Lumpur 50603, Malaysia
[2] Univ Malaya, Dept Physiol, Fac Med, Kuala Lumpur 50603, Malaysia
[3] Univ Malaya, Dept Restorat Dent, Fac Dent, Kuala Lumpur 50603, Malaysia
关键词
applications; biocompatibility; biomaterials; biomedical applications; polyesters; CYTOCOMPATIBILITY; BIOCOMPATIBILITY; FUMARATE); APOPTOSIS; MATRICES; SURFACE; CELLS;
D O I
10.1002/app.43711
中图分类号
O63 [高分子化学(高聚物)];
学科分类号
070305 ; 080501 ; 081704 ;
摘要
Bone tissue engineering offers high hopes in reconstructing bone defects that result from trauma, infection, tumors, and other conditions. However, there remains a need for novel scaffold materials that can effectively stimulate ossification with appropriate functional properties. Therefore, a novel injectable, biodegradable, and biocompatible scaffold made by incorporating modified poly(caprolactone trifumarate) (PCLTF) with embedded gelatin microparticles (GMPs) as porogen is developed. Specifically, in vitro and in vivo tests were carried out. For the latter, to determine the osteogenic ability of PCLTF-GMPs scaffolds, and to characterize bone-formation, these scaffolds were implanted into critical-sized defects of New Zealand white rabbit craniums. Field Emission Scanning Electron Microscope (FESEM) demonstrated cells of varying shapes attached to the scaffold surface in vitro. The PCLTF-GMPs demonstrated improved biocompatibility in vivo. Polyfluorochrome tracers detected bone growth occurring in the PCLTF-GMPs filled defects. By incorporating PCLTF with GMPs, we have fabricated a promising self-crosslinkable biocompatible and osteoconducive scaffold for bone tissue engineering. (c) 2016 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2016, 133, 43711.
引用
收藏
页数:15
相关论文
共 50 条
  • [1] Electrospun gelatin/poly(ε-caprolactone) fibrous scaffold modified with calcium phosphate for bone tissue engineering
    Rajzer, Izabella
    Menaszek, Elzbieta
    Kwiatkowski, Ryszard
    Planell, Josep A.
    Castano, Oscar
    [J]. MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS, 2014, 44 : 183 - 190
  • [2] An injectable poly(caprolactone trifumarate-gelatin microparticles) (PCLTF-GMPs) scaffold for irregular bone defects: Physical and mechanical characteristics
    Al-Namnam, Nisreen Mohammed
    Kutty, Muralithran Govindan
    Chai, Wen Lin
    Ha, Kien Oon
    Kim, Kah Hwi
    Siar, Chong Huat
    Ngeow, Wei Cheong
    [J]. MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS, 2017, 72 : 332 - 340
  • [3] Poly(caprolactone) Shape Memory Scaffold for Bone Tissue Engineering
    Baker, R. M.
    Henderson, J. H.
    Mather, P. T.
    [J]. 2012 38TH ANNUAL NORTHEAST BIOENGINEERING CONFERENCE (NEBEC), 2012, : 309 - 310
  • [4] A Viscoelastic Study of Poly(ε-Caprolactone) Microsphere Sintered Bone Tissue Engineering Scaffold
    Shahin-Shamsabadi, Alireza
    Hashemi, Ata
    Tahriri, Mohammadreza
    [J]. JOURNAL OF MEDICAL AND BIOLOGICAL ENGINEERING, 2018, 38 (03) : 359 - 369
  • [5] Fabrication of chitosan/poly(caprolactone) nanofibrous scaffold for bone and skin tissue engineering
    Shalumon, K. T.
    Anulekha, K. H.
    Chennazhi, K. P.
    Tamura, H.
    Nair, S. V.
    Jayakumar, R.
    [J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2011, 48 (04) : 571 - 576
  • [6] A Viscoelastic Study of Poly(ε-Caprolactone) Microsphere Sintered Bone Tissue Engineering Scaffold
    Alireza Shahin-Shamsabadi
    Ata Hashemi
    Mohammadreza Tahriri
    [J]. Journal of Medical and Biological Engineering, 2018, 38 : 359 - 369
  • [7] Alginate-gelatin microparticles as injectable scaffold for myocardial tissue engineering
    Rosellini, E.
    Barbani, N.
    Tinti, E.
    Cristallini, C.
    [J]. JOURNAL OF APPLIED BIOMATERIALS & BIOMECHANICS, 2009, 7 (01) : 69 - 69
  • [8] Electrospun poly(ε-caprolactone)-gelatin mats for tissue engineering scaffolding
    Bianco, A.
    Del Gaudio, C.
    Cacciotti, I.
    [J]. JOURNAL OF APPLIED BIOMATERIALS & BIOMECHANICS, 2009, 7 (01) : 55 - 55
  • [9] Demineralized bone matrix gelatin as scaffold for tissue engineering
    Pan, Yong
    Dong, Shiwu
    Hao, Yong
    Chu, Tongwei
    Li, Changqing
    Zhang, Zhengfeng
    Zhou, Yue
    [J]. AFRICAN JOURNAL OF MICROBIOLOGY RESEARCH, 2010, 4 (09): : 865 - 870
  • [10] The surface properties of the composite poly (ε-caprolactone) scaffold for tissue engineering
    Intarat, Pantanee
    Oonkhanond, Bovornlak
    [J]. TISSUE ENGINEERING, 2006, 12 (04): : 996 - 996