Alteromonas oceanisediminis sp. nov., isolated from deep-sea sediment

A Gram-stain-negative, aerobic and rod-shaped bacterium, designated strain SM 2104(T), was isolated from a deep-sea sediment sample collected from the Southwest Indian Ocean. Strain SM 2104(T) grew at 10-37 degrees C (optimum at 25 degrees C), and with 1.0-9.0% (w/v, optimum with 2-4%) NaCl. It hydrolyzed starch, tween 80 and gelatin but did not reduced nitrate to nitrite. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain SM 2104(T) was affiliated with the genus Alteromonas, sharing the highest 16S rRNA gene sequence similarities with type strains of Alteromonas flava (97.5%) and Alteromonas facilis (97.4%) and forming a distinct clade together with the two Alteromonas species. The digital DNA-DNA hybridization and average nucleotide identity values between strain SM 2104( T) and type strains of Alteromonas flava and Alteromonas facilis were below 14.5%, and 71.0%, respectively. The major fatty acids of strain SM 2104(T) were summed feature 3 (C-16:1 omega 6c/C-16:1 omega 7c), C-16:0 and summed feature 8 (C-18:1 omega 7c/C-18:1 omega 6c). The major polar lipids of strain SM 2104(T) were phosphatidylethanolamine and phosphatidylglycerol and the only respiratory quinone of strain SM 2104(T) was ubiquinone-8. The genomic DNA G + C content of strain SM 2104(T) was 48.0%. On the basis of the phylogenetic, phenotypic, chemotaxonomic and genomic analyses presented in this study, strain SM 2104(T) is considered to represent a novel species within the genus Alteromonas, for which the name Alteromonas oceansediminis sp. nov. is proposed. The type strain is SM 2104(T) (= CCTCC AB 2021121(T) = KCTC 82867(T)).