Refined solution structure of the dimeric N-terminal HHCC domain of HIV-2 integrase

被引:19
|
作者
Eijkelenboom, APAM
van den Ent, FMI
Wechselberger, R
Plasterk, RHA
Kaptein, R
Boelens, R [1 ]
机构
[1] Univ Utrecht, Bijovet Ctr Biomol Res, NL-3584 CH Utrecht, Netherlands
[2] Netherlands Canc Inst, Div Mol Biol, NL-1066 CX Amsterdam, Netherlands
关键词
helix-turn-helix motif; HIV; integrase; protein dimer; protein structure; zinc-binding domain;
D O I
10.1023/A:1008342312269
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The solution structure of the dimeric N-terminal domain of HIV-2 integrase (residues 1-55, named IN1-55) has been determined using NMR spectroscopy. The structure of the monomer, which was already reported previously [Eijkelenboom et al. (1997) Curr. Biol., 7, 739-746], consists of four alpha -helices and is well defined. Helices alpha1, alpha2 and alpha3 form a three-helix bundle that is stabilized by zinc binding to His12, His16, Cys40 and Cys43. The dimer interface is formed by the N-terminal tail and the first half of helix alpha3. The orientation of the two monomeric units with respect to each other shows considerable variation. N-15 relaxation studies have been used to characterize the nature of the intermonomeric disorder. Comparison of the dimer interface with that of the well-defined dimer interface of HIV-1 IN1-55 shows that the latter is stabilized by additional hydrophobic interactions and a potential salt bridge. Similar interactions cannot be formed in HIV-2 IN1-55 [Cai et al. (1997) Nat. Struct. Biol., 4, 567-577], where the corresponding residues are positively charged and neutral ones.
引用
收藏
页码:119 / 128
页数:10
相关论文
共 50 条
  • [1] Refined solution structure of the dimeric N-terminal HHCC domain of HIV-2 integrase
    Astrid P.A.M. Eijkelenboom
    Fusinita M.I. van den Ent
    Rainer Wechselberger
    Ronald H.A. Plasterk
    Robert Kaptein
    Rolf Boelens
    [J]. Journal of Biomolecular NMR, 2000, 18 : 119 - 128
  • [2] The solution structure of the amino-terminal HHCC domain of HIV-2 integrase: a three-helix bundle stabilized by zinc
    Eijkelenboom, APAM
    vandenEnt, FMI
    Vos, A
    Doreleijers, JF
    Hard, K
    Tullius, TD
    Plasterk, RHA
    Kaptein, R
    Boelens, R
    [J]. CURRENT BIOLOGY, 1997, 7 (10) : 739 - 746
  • [3] Solution structure of the N-terminal zinc binding domain of HIV-1 integrase
    Cai, ML
    Zheng, RL
    Caffrey, M
    Craigie, R
    Clore, GM
    Gronenborn, AM
    [J]. NATURE STRUCTURAL BIOLOGY, 1997, 4 (07) : 567 - 577
  • [4] Solution structure of the N-terminal zinc binding domain of HIV-1 integrase
    [J]. Nature Structural Biology, 1997, 4 : 839 - 840
  • [5] Solution structure of the N-terminal zinc binding domain of HIV-1 integrase
    Mengli Cai
    Ronglan Zheng
    Michael Caffrey
    Robert Craigie
    G. Marius Clore
    Angela M. Gronenborn
    [J]. Nature Structural Biology, 1997, 4 : 567 - 577
  • [6] X-ray absorption spectroscopic studies of zinc in the N-terminal domain of HIV-2 integrase and model compounds
    Feiters, MC
    Eijkelenboom, APAM
    Nolting, HF
    Krebs, B
    van den Ent, FMI
    Plasterk, RHA
    Kaptein, R
    Boelens, R
    [J]. JOURNAL OF SYNCHROTRON RADIATION, 2003, 10 : 86 - 95
  • [7] Solution structure of the N-terminal zinc binding domain of HIV-1 integrase (vol 4, pg 567, 1997)
    Cai, ML
    Zheng, RL
    Caffrey, M
    Craigie, R
    Clore, GM
    Gronenborn, AM
    [J]. NATURE STRUCTURAL BIOLOGY, 1997, 4 (10) : 839 - 840
  • [8] The solution structure of the N-terminal domain of riboflavin synthase
    Truffault, V
    Coles, M
    Diercks, T
    Abelmann, K
    Eberhardt, S
    Lüttgen, H
    Bacher, A
    Kessler, H
    [J]. JOURNAL OF MOLECULAR BIOLOGY, 2001, 309 (04) : 949 - 960
  • [9] Solution structure of the His12→Cys mutant of the N-terminal zinc binding domain of HIV-1 integrase complexed to cadmium
    Cai, ML
    Huang, Y
    Caffrey, M
    Zheng, RL
    Craigie, R
    Clore, GM
    Gronenborn, AM
    [J]. PROTEIN SCIENCE, 1998, 7 (12) : 2669 - 2674
  • [10] Solution structure of the COMMD1 N-terminal domain
    Sommerhalter, Monika
    Zhang, Yongbo
    Rosenzweig, Amy C.
    [J]. JOURNAL OF MOLECULAR BIOLOGY, 2007, 365 (03) : 715 - 721