Optical and electrical properties of bacteriorhodopsin Langmuir-Blodgett films: II. D96N mutant and its 4-keto and 9-demethyl retinal analogs

被引：8

作者：

Weetall, HH ^{[1
]}

Druzhko, AB

Samuelson, LA

de Lera, AR

Alvarez, R

机构：

[1] Natl Inst Stand & Technol, Div Biotechnol, Gaithersburg, MD 20899 USA

[2] USA, Natick Res Dev & Engn Ctr, Div Biotechnol, Natick, MA 01760 USA

[3] Univ Virgo, Fac Ciencias, Dept Quim Pura & Aplicada, Virgo 36200, Spain

来源：

BIOELECTROCHEMISTRY AND BIOENERGETICS | 1997年 / 44卷 / 01期

关键词：

Langmuir-Blodgett films; bacteriorhodopsin; purple membranes; photocurrents;

D O I：

10.1016/S0302-4598(97)00069-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Langmuir-Blodgett films prepared from purple membranes of the D96N mutant and two of its chromophore analogs (4-keto-retinal and 9-demethyl-retinal) were characterized for M-state kinetics and photocurrent production. These studies indicated that unlike the wild-type (WT) and its identical chromophore analogs, the photocurrent transients were unusual, showing proton release at pH 3.0. Several possible explanations for these results are considered. (C) 1997 Elsevier Science S.A.

引用

页码：37 / 43

页数：7

共 5 条

[1] Photoinduced transformation of 4-keto-bacteriorhodopsin gelatin films based on both wild type and D96N mutant
Druzhko, AB
Weetall, HH
BIOPHYSICAL JOURNAL, 1997, 72 (02) : MAMJ6 - MAMJ6
[2] Optical and electrical properties of oriented purple membrane films containing D96N bacteriorhodopsin at different humidities
Lukashev, EP
Kononenko, AA
Rubin, AB
BIOLOGICHESKIE MEMBRANY, 1996, 13 (04): : 341 - 353
[3] Neutron reflection from 2,4-bis(4-(N-methyl-N-octylamino)phenyl)squaraine at the air-water interface and the linear and nonlinear optical properties of its Langmuir-Blodgett and spun-coated films
Ashwell, GJ
Wong, GMS
Bucknall, DG
Bahra, GS
Brown, CR
LANGMUIR, 1997, 13 (06) : 1629 - 1633
[4] 2,4-bis[4-(N,N-dibutylamino)phenyl]squaraine: X-ray crystal structure of a centrosymmetric dye and the second-order non-linear optical properties of its non-centrosymmetric Langmuir-Blodgett films
Ashwell, GJ
Bahra, GS
Brown, CR
Hamilton, DG
Kennard, CHL
Lynch, DE
JOURNAL OF MATERIALS CHEMISTRY, 1996, 6 (01) : 23 - 26
[5] Second-order non-linear optical properties of Langmuir-Blodgett films of a new azo compound (p-NO2)C6H4N=NC10H6O(CH2)(3)N(CH3)(2)C18H37Br and its europium complex
Xia, WS
Huang, CH
Cheng, TR
Gan, LB
Zhao, XS
Yu, AC
JOURNAL OF MATERIALS CHEMISTRY, 1996, 6 (09) : 1493 - 1495

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