A bio-guided approach for the development of a chestnut-based proanthocyanidin-enriched nutraceutical with potential anti-gastritis properties

被引：28

作者：

Sangiovanni, Enrico ^{[1
]}

Piazza, Stefano ^{[1
]}

Vrhovsek, Urska ^{[2
]}

Fumagalli, Marco ^{[1
]}

Khalilpour, Saba ^{[1
]}

Masuero, Domenico ^{[2
]}

Di Lorenzo, Chiara ^{[1
]}

Colombo, Luca ^{[3
]}

Mattivi, Fulvio ^{[2
]}

De Fabiani, Emma ^{[1
]}

Dell'Agli, Mario ^{[1
]}

机构：

[1] Univ Milan, Dept Pharmacol & Biomol Sci, Milan, Italy

[2] E Mach Fdn, Food Qual & Nutr Dept, San Michele All Adige, TN, Italy

[3] Soc Cooperat Agr Varese, Consorzio Castanicoltori Brinzio Orino & Castello, Varese, Italy

来源：

PHARMACOLOGICAL RESEARCH | 2018年 / 134卷

关键词：

Chestnut; Gastric inflammation; Proanthocyanidins; interleukin-8; NECROSIS-FACTOR-ALPHA; CASTANEA-SATIVA MILL; HELICOBACTER-PYLORI; INTERLEUKIN-8; PRODUCTION; TANNINS; COCOA; PROCYANIDINS; EXPRESSION; PATHWAYS; CAPACITY;

D O I：

10.1016/j.phrs.2018.06.016

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Gastritis is a widely spread inflammatory disease, mostly caused by Helicobacter pylori infection. Release of IL-8 by the stomach epithelium is a hallmark of gastritis and contributes to the amplification of the inflammatory state. Pharmacological modulation of IL-8 release is a strategy to relieve gastric inflammation and prevent more severe clinical outcomes. In search of nutraceuticals with potential anti-gastritis properties we used a bio-guided approach based on IL-8 secretion by gastric cells to characterize extracts from the fruits of different chestnut varieties. We found that the ability to inhibit IL-8 secretion correlated with the amount of proanthocyanidins and was associated to the not edible parts of chestnut in all the tested varieties. We also found that the anti-inflammatory activity is preserved upon mild thermal treatment and after in vitro simulated gastric digestion. By combining a robust bio-guided approach with a comprehensive analysis of the tannin fraction of chestnut extracts, we provide evidence for the potential use of chestnut-based nutraceuticals in human gastritis. The bioactive components of chestnut fruits inhibit IL-8 secretion by impairing NF-kappa B signaling and by other mechanisms, thus opening new applications of proanthocyanidins for inflammation-based diseases.

引用

页码：145 / 155

页数：11