Determination of glucosamine in nutritional supplements by reversed-phase ion-pairing HPLC

被引：20
|
作者：
Way, WK [1 ]
Gibson, KG [1 ]
Breite, AG [1 ]
机构：
[1] Chemir Polytech Labs Inc, Maryland Hts, MO 63043 USA
来源：
JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES | 2000年 / 23卷 / 18期
关键词：
D O I：
10.1081/JLC-100101238
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A reverse-phase high performance liquid chromatography (HPLC) method for the determination of glucosamine in nutritional supplements was developed and validated based on USP guidelines. Separations were performed using a MetaChem Inertsil ODS-3 column with an isocratic mobile phase consisting of 10% Methanol, 90% 0.005M octanesulfonate, pH = 2.1. Glucosamine was detected using a refractive index (RI) detector. The octanesulfonate ion pairing agent allowed for retention on a C-18 column. The method proved highly reliable with respect to standard performance characteristics. The method allows for convenient quality control in the growing nutritional supplement market.
引用
收藏
页码：2861 / 2871
页数：11
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