DNA-polyfluorophore excimers as sensitive reporters for esterases and lipases

被引：29

作者：

Dai, Nan ^{[1
]}

Teo, Yin Nah ^{[1
]}

Kool, Eric T. ^{[1
]}

机构：

[1] Stanford Univ, Dept Chem, Stanford, CA 94305 USA

来源：

CHEMICAL COMMUNICATIONS | 2010年 / 46卷 / 08期

基金：

美国国家卫生研究院;

关键词：

INTRAMOLECULAR DIMERS; SEQUENCE DEPENDENCE; FLUORESCENCE; LABELS; INHIBITORS; BACKBONE; PRODRUGS; ENZYMES; PROBES;

D O I：

10.1039/b926338a

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

DNA-scaffolded oligodeoxyriboside fluorophores (ODFs) were used as the reporters in turn-on sensing of enzymatic bond-cleaving activity. A tetramer ODF of pyrene deoxynucleosides displayed high quenching efficiency when conjugated via ester linkages with a dabcyl quencher, and yielded large signal increases with several enzymes in vitro and in intact human cells.

引用

页码：1221 / 1223

页数：3

共 9 条

[1] Polyfluorophore Excimers and Exciplexes as FRET Donors in DNA
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BIOCONJUGATE CHEMISTRY, 2009, 20 (12) : 2371 - 2380
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[8] HORMONE-SENSITIVE LIPASE IS CLOSELY-RELATED TO SEVERAL BACTERIAL PROTEINS, AND DISTANTLY RELATED TO ACETYLCHOLINESTERASE AND LIPOPROTEIN-LIPASE - IDENTIFICATION OF A SUPERFAMILY OF ESTERASES AND LIPASES
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