Gentamicin-induced spiral ganglion cell death: Apoptosis mediated by ROS and the JNK signaling pathway

被引:34
|
作者
Jeong, Sung-Wook [1 ]
Kim, Lee-Suk [1 ]
Hur, Daeyoung [3 ]
Bae, Woo-Yong [1 ]
Kim, Jae-Ryong [2 ]
Lee, Jae-Hoon [1 ]
机构
[1] Dong A Univ, Coll Med, Dept Otolaryngol Head & Neck Surg, Pusan 602715, South Korea
[2] Inje Univ, Coll Med, Dept Otolaryngol Head & Neck Surg, Pusan, South Korea
[3] Inje Univ, Coll Med, Dept Anat & Tumor Immunol, Pusan, South Korea
关键词
Spiral ganglion cell; apoptosis; reactive oxygen species; JNK; c-Jun; gentamicin; REACTIVE OXYGEN; SYMPATHETIC NEURONS; OXIDATIVE STRESS; GUINEA-PIG; ACTIVATION;
D O I
10.3109/00016480903428200
中图分类号
R76 [耳鼻咽喉科学];
学科分类号
100213 ;
摘要
Conclusion: Reactive oxygen species (ROS) and the c-Jun N-terminal kinase (JNK) signaling pathway may be involved in secondary apoptosis of spiral ganglion cells (SGCs) induced by intracochlear gentamicin injection. Objectives: The purpose of this study was to ascertain the role of ROS and the JNK signaling pathway in secondary apoptosis of SGCs induced by intracochlear gentamicin treatment. Methods: Gentamicin (40 mg/ml) was injected into the cochlea of guinea pigs (n = 18) to destroy the hair cells and induce secondary apoptosis of SGCs. At 1 (n = 6), 2 (n = 6), and 3 (n = 6) weeks after gentamicin treatment, the cochleas were removed and stained with hematoxylin and eosin, and terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick-end labeling to observe the morphologic changes and apoptosis of SGCs. A dihydroethidium (DHE) assay was performed to detect ROS generation, and RT-PCR and Western blot analysis were used to assess the expression of Fas ligand (FasL), JNK, and c-Jun. Results: After gentamicin was injected into the cochlea, apoptosis and progressive loss of SGCs were observed. RT-PCR and Western blot analysis showed increased expression of FasL after gentamicin treatment. ROS generation detected by DHE fluorescence increased progressively, and the expression of JNK, phospho-JNK, c-Jun, and phospho-c-Jun also increased.
引用
收藏
页码:670 / 678
页数:9
相关论文
共 50 条
  • [1] Gentamicin-induced hair cell death is not dependent on the apoptosis receptor Fas
    Bodmer, D
    Brors, D
    Pak, K
    Bodmer, M
    Ryan, AF
    [J]. LARYNGOSCOPE, 2003, 113 (03): : 452 - 455
  • [2] Rapamycin Protects Spiral Ganglion Neurons from Gentamicin-Induced Degeneration In Vitro
    Guo, Shasha
    Xu, Nana
    Chen, Peng
    Liu, Ying
    Qi, Xiaofei
    Liu, Sheng
    Li, Cuixian
    Tang, Jie
    [J]. JARO-JOURNAL OF THE ASSOCIATION FOR RESEARCH IN OTOLARYNGOLOGY, 2019, 20 (05): : 475 - 487
  • [3] Rapamycin Protects Spiral Ganglion Neurons from Gentamicin-Induced Degeneration In Vitro
    Shasha Guo
    Nana Xu
    Peng Chen
    Ying Liu
    Xiaofei Qi
    Sheng Liu
    Cuixian Li
    Jie Tang
    [J]. Journal of the Association for Research in Otolaryngology, 2019, 20 : 475 - 487
  • [4] Apoptosis-Inducing Factor Is Involved in Gentamicin-Induced Vestibular Hair Cell Death
    Zhang, Daogong
    Fan, Zhaomin
    Han, Yuechen
    Bai, Xiaohui
    Liu, Wenwen
    Lu, Sumei
    Wang, Mingming
    Xu, Lei
    Luo, Jianfen
    Li, Jianfeng
    Wang, Haibo
    [J]. ORL-JOURNAL FOR OTO-RHINO-LARYNGOLOGY HEAD AND NECK SURGERY, 2012, 74 (01): : 1 - 5
  • [5] Guggulsterone-induced apoptosis in cholangiocarcinoma cells through ROS/JNK signaling pathway
    Zhong, Fei
    Tong, Zhu-Ting
    Fan, Lu-Lu
    Zha, Li-Xia
    Wang, Fang
    Yao, Meng-Qun
    Gu, Kang-Sheng
    Cao, Yun-Xia
    [J]. AMERICAN JOURNAL OF CANCER RESEARCH, 2016, 6 (02): : 226 - 237
  • [6] Induction of ROS-mediated cell death and activation of the JNK pathway by a sulfonamide derivative
    Ahmad, Rehan
    Vaali-Mohammed, Mansoor-Ali
    Elwatidy, Mohammed
    Al-Obeed, Omar
    Al-Khayal, Khayal
    Eldehna, Wagdy M.
    Abdel-Aziz, Hatem A.
    Alafeefy, Ahmed
    Abdulla, Maha
    [J]. INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE, 2019, 44 (04) : 1552 - 1562
  • [7] Propranolol induced apoptosis and autophagy via the ROS/JNK signaling pathway in Human Ovarian Cancer
    Zhao, Shujun
    Fan, Suzhen
    Shi, Yanyu
    Ren, Hongyan
    Hong, Hanqing
    Gao, Xiang
    Zhang, Min
    Qin, Qiaohong
    Li, Hongyu
    [J]. JOURNAL OF CANCER, 2020, 11 (20): : 5900 - 5910
  • [8] DHA ameliorates MeHg-induced PC12 cell apoptosis by inhibiting the ROS/JNK signaling pathway
    Zhang, Hong
    Wang, Susu
    Wang, Yaqian
    Lu, Anxin
    Hu, Chunping
    Yan, Chonghuai
    [J]. MOLECULAR MEDICINE REPORTS, 2021, 24 (02)
  • [9] Isosorbide delays gentamicin-induced vestibular sensory cell death
    Takumida, M
    Anniko, M
    [J]. ORL-JOURNAL FOR OTO-RHINO-LARYNGOLOGY AND ITS RELATED SPECIALTIES, 2005, 67 (05): : 276 - 281
  • [10] Pristimerin Induces Autophagy-Mediated Cell Death in K562 Cells through the ROS/JNK Signaling Pathway
    Liu, Yingxiang
    Ren, Ziting
    Li, Xiang
    Zhong, Jing
    Bi, Yun
    Li, Rui
    Zhao, Qun
    Yu, Xianjun
    [J]. CHEMISTRY & BIODIVERSITY, 2019, 16 (08)