Multiplex detection of mutations in clinical isolates of rifampin-resistant Mycobacterium tuberculosis by short oligonucleotide Ligation assay on DNA chips

被引：8

作者：

Deng, JY

Zhang, XE ^{[1
]}

Lu, HB

Liu, Q

Zhang, ZP

Zhou, YF

Xie, WH

Fu, ZJ

机构：

[1] Chinese Acad Sci, Wuhan Inst Virol, Wuhan 430071, Peoples R China

[2] Chinese Acad Sci, Wuhan Inst Hydrobiol, Wuhan 430071, Peoples R China

[3] Wuhan TB Prevent & Cure Inst, Wuhan, Peoples R China

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2004年 / 42卷 / 10期

关键词：

D O I：

10.1128/JCM.42.10.4850-4852.2004

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A new approach, short-oligonucleotide-ligation assay on DNA chip (SOLAC), is developed to detect mutations in rifampin-resistant Mycobacterium tuberculosis. The method needs only four common probes to detect 15 mutational variants of the rpoB gene within 12 h. Fifty-five rifampin-resistant M. tuberculosis isolates were analyzed, resulting in 87.3% accuracy and 83.6% concordance relative to DNA sequencing.

引用

页码：4850 / 4852

页数：3

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