High-resolution tomographic diffractive microscopy of biological samples

被引：55
|
作者：
Simon, Bertrand [1 ]
Debailleul, Matthieu [1 ]
Beghin, Anne [2 ]
Tourneur, Yves [2 ]
Haeberle, Olivier [1 ]
机构：
[1] Univ Haute Alsace, IUT Mulhouse, EA2332, Lab MIPS, F-68093 Mulhouse, France
[2] Univ Lyon 1, Ctr Commun Quantimetrie, F-69373 Lyon, France
来源：
JOURNAL OF BIOPHOTONICS | 2010年 / 3卷 / 07期
关键词：
3D microscopy; holography; tomographic diffractive microscopy; biological analysis;
D O I：
10.1002/jbio.200900094
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The authors have developed tomographic diffractive microscope that combines microholography with illumination from an angular synthetic aperture. It images specimens relative to their complex index of refraction distribution (index and absorption) and permits imaging of unlabelled specimens with high lateral resolution. The authors now study its use for biological applications and imaged several preparations with fluorescence confocal microscopy and tomographic diffractive microscopy. The results highlights some interesting features of this instrument, which should attract the interest of biologists for this new technique. [GRAPHICS] Multimodal imaging of human influenza infected A549 cells.
引用
收藏
页码：462 / 467
页数：6
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