Synthesis of polyacrylamide supports and immobilization of Candida cylindracea lipase

被引：0
|
作者：
Xu, HX [1 ]
Wang, GC [1 ]
Li, MQ [1 ]
He, BL [1 ]
机构：
[1] NANKAI UNIV,STATE KEY LAB FUNCT POLYMER MAT ADSORPT & SEPARAT,INST POLYMER CHEM,TIANJIN 300071,PEOPLES R CHINA
来源：
PROGRESS IN NATURAL SCIENCE | 1997年 / 7卷 / 01期
关键词：
polyacrylamide; poly(methyl acrylate); Candida cylindracea; immobilized lipase;
D O I：
暂无
中图分类号：
T [工业技术];
学科分类号：
08 ;
摘要：
The polyacrylamide supports with amino groups were synthesized by aminating poly(methyl acrylate) with polyamine. Candida cylindracea lipase (CCL) was immobilized on these supports and the activity of ester hydrolysis catalyzed by immobilized lipase was investigated. Some rules were found for the influence of aminating conditions and support structure on the immobilization of CCL. CCL immobilized on some supports has high enzymatic activity. The effects of pH, ionic strength of substrate solution and reaction temperature on the activity of dissolved CCL were compared with those on immobilized CCL. The operational stability oi immobilized CCL was also studied.
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页码：46 / 50
页数：5
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