Selection of formulated sediment and feeding condition for 10-day spiked-sediment toxicity test with estuarine amphipod Grandidierella japonica

This study was performed to select formulated sediment with appropriate feeding conditions for the evaluation of estuarine sediment toxicity. Grandidierella japonica, which inhabits seawater worldwide, was examined for 10-day survival, and the repeatability was evaluated under nine test conditions. The formulated sediment feeding condition combined with the highest survival rate was applied to evaluate fluoranthene toxicity in the sediment. The KB-T10 feeding condition showed the highest survival rate of 93.5%, with sufficient repeatability (4.6%). In the fluoranthene spiked-sediment toxicity test, the estimated LC50 (95% significance level) was successfully achieved under the KB-T10 condition but not under the OE-T10 condition. Therefore, we propose the use of TetraMin (R) (1 mg/org./day) and Kemble sediment in 10-day static spiked-sediment toxicity tests with G. japonica. Further improvements are needed for OECD sediment in seawater to increase the survival rate and prevent chemical loss due to overlying water renewal.