Evaluation of effects of prenatal exposure to the cyanide and thiocyanate in wistar rats

A study was performed at term and at weaning to verify the toxic effects of the prenatal exposure to potassium cyanide (KCN) and potassium thiocyanate (KSCN) in pregnant Wistar rats. Females received daily in drinking water the doses: 1, 3 and 30 mg KCN/kg or 0.8, 2.4 and 24 mg KSCN/kg from GD 6 to GD 20 and were euthanized on GD 20 (trial A) or one day after weaning (trial 13). Skeletal and visceral analyses of the fetuses (trial A) were performed and samples of blood and different organs, from both dams (trials A and B) and weaned pups (trial A), were collected in order to perform the biochemical evaluation and histopathology. Results showed high thiocyanate levels in dams of the different experimental groups from both trials. The intensity of the histological lesions observed in dams of trial B was similar to that of trial A, except those lesions found in the pancreas. The histopathological study of this organ revealed loss of cells in the Islets of Langerhans from dams of trial A which received the highest dose of cyanide (CN). There was an increase in the number of biliary ducts in animals treated with the highest doses of both thiocyanate and cyanide. The histopathological study of the spleen and the lungs of experimental and control groups did not reveal any significant alteration. In relation to fetuses (trial A), the visceral and skeletal evaluations did not reveal any significant malformation; on the other hand, pups from trial B showed some histological alterations similar to those observed in their dams. It is concluded that the cyanide and/or thiocyanate promoted toxic effects in the fetuses some of which could also be observed at weaning. (c) 2007 Elsevier Inc. All rights reserved.