Tetracycline-inducible system for photoreceptor-specific gene expression

被引:1
|
作者
Chang, MA
Horner, JW
Conklin, BR
DePinho, RA
Bok, D
Zack, DJ
机构
[1] Univ Calif Los Angeles, Brain Res Inst, Jules Stein Eye Inst, Los Angeles, CA 90024 USA
[2] Univ Calif Los Angeles, Brain Res Inst, Dept Neurobiol, Los Angeles, CA 90024 USA
[3] Univ Calif San Francisco, Gladstone Inst Cardiovasc Dis, San Francisco, CA 94143 USA
[4] Univ Calif San Francisco, Dept Med, San Francisco, CA USA
[5] Univ Calif San Francisco, Dept Pharmacol, San Francisco, CA 94143 USA
[6] Albert Einstein Coll Med, Dept Cell Biol, Bronx, NY 10467 USA
[7] Johns Hopkins Univ, Sch Med, Wilmer Eye Inst, Baltimore, MD 21205 USA
[8] Johns Hopkins Univ, Sch Med, Dept Mol Biol & Genet, Baltimore, MD 21205 USA
[9] Johns Hopkins Univ, Sch Med, Dept Neurosci, Baltimore, MD 21205 USA
关键词
D O I
暂无
中图分类号
R77 [眼科学];
学科分类号
100212 ;
摘要
PURPOSE. TO develop a system for inducible photoreceptor-specific gene expression in transgenic mice. The tetracycline regulatory system was chosen because it possesses the useful property of direct control of gene expression through use of an exogenous agent, doxycycline, a tetracycline derivative. METHODS. Transgenic mice were generated that carried the reverse tetracycline-controlled transactivator under the control of the photoreceptor-specific promoters for rhodopsin and interphotoreceptor retinoid-binding protein. These animals were crossed with transgenic mice carrying the lad, reporter gene under control of the tetracycline operator cassette, creating doubly transgenic mice. Doxycycline was administered to induce expression of the reporter gene. Reporter assays were then performed to evaluate lacZ expression. RESULTS. Doxycycline administration led to phororeceptor-specific expression of the lacZ reporter gene in the doubly transgenic mice. X-gal staining was restricted to photoreceptor inner segments and synaptic termini. Induction could be achieved by addition of the drug to the animals' drinking water or by intravitreal injection. Induction was noted within 24 hours of doxcycline administration. Because of variability among animals, there was an approximate correlation, but not a clean dose-response curve relating drug dose to level of reporter expression. CONCLUSIONS. ii transgenic system for inducible photoreceptor-specific gene expression has been developed. This system is currently being exploited to study the effects of regulated expression of genes of biological interest.
引用
收藏
页码:4281 / 4287
页数:7
相关论文
共 50 条
  • [1] Tetracycline-inducible gene expression system in Leishmania mexicana
    Kraeva, Natalya
    Ishemgulova, Aygul
    Lukes, Julius
    Yurchenko, Vyacheslav
    [J]. MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 2014, 198 (01) : 11 - 13
  • [2] A tetracycline-inducible gene expression system in Entamoeba histolytica
    Ramakrishnan, G
    Vines, RR
    Mann, BJ
    Petri, WA
    [J]. MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1997, 84 (01) : 93 - 100
  • [3] First report of a tetracycline-inducible gene expression system for mollicutes
    Breton, Marc
    Sagne, Evelyne
    Duret, Sybille
    Beven, Laure
    Citti, Christine
    Renaudin, Joel
    [J]. MICROBIOLOGY-SGM, 2010, 156 : 198 - 205
  • [5] Tetracycline-inducible gene expression in Trichomonas vaginalis
    Ortiz, D
    Johnson, PJ
    [J]. MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 2003, 128 (01) : 43 - 49
  • [6] Tetracycline-inducible system for regulation of skeletal muscle-specific gene expression in transgenic mice
    Grill, MA
    Bales, MA
    Fought, AN
    Rosburg, KC
    Munger, SJ
    Antin, PB
    [J]. TRANSGENIC RESEARCH, 2003, 12 (01) : 33 - 43
  • [7] Tetracycline-Inducible System for Regulation of Skeletal Muscle-Specific Gene Expression in Transgenic Mice
    Mischala A. Grill
    Mark A. Bales
    Amber N. Fought
    Kristopher C. Rosburg
    Stephanie J. Munger
    Parker B. Antin
    [J]. Transgenic Research, 2003, 12 : 33 - 43
  • [8] Tetracycline-inducible gene expression and gene deletion in Candida albicans
    Park, YN
    Morschhäuser, J
    [J]. EUKARYOTIC CELL, 2005, 4 (08) : 1328 - 1342
  • [9] Construction of a tapetum-specific and tetracycline-inducible system
    Tang, SY
    Yu, BL
    Niu, HL
    Zhang, LM
    Sun, YR
    Li, WB
    [J]. ACTA BOTANICA SINICA, 2003, 45 (01): : 64 - 67
  • [10] On the use of the T-REx™ tetracycline-inducible gene expression system in vivo
    Dobrovolsky, Vasily N.
    Heflich, Robert H.
    [J]. BIOTECHNOLOGY AND BIOENGINEERING, 2007, 98 (03) : 719 - 723