Transposon-based approaches to identify essential bacterial genes

被引：69
|
作者：
Judson, N [1 ]
Mekalanos, JJ [1 ]
机构：
[1] Harvard Univ, Sch Med, Dept Microbiol & Mol Genet, Boston, MA 02115 USA
来源：
TRENDS IN MICROBIOLOGY | 2000年 / 8卷 / 11期
关键词：
D O I：
10.1016/S0966-842X(00)01865-5
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Transposons are a powerful tool for identifying genes essential for bacterial viability. The availability of many bacterial genome sequences and the large number of genes of unknown function therein have inspired the generation of a variety of different approaches. These methods are described and their advantages and disadvantages are discussed.
引用
收藏
页码：521 / 526
页数：6
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