Triplex molecular beacons as modular probes for DNA detection

被引：122
|
作者：
Grossmann, Tom N. [1 ]
Roeglin, Lars [1 ]
Seitz, Oliver [1 ]
机构：
[1] Humboldt Univ, Inst Chem, D-12489 Berlin, Germany
来源：
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2007年 / 46卷 / 27期
关键词：
bioanalysis; DNA recognition; fluorescent probes; FRET (fluorescence resonant energy transfer); molecular beacons;
D O I：
10.1002/anie.200700289
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
(Figure Presented) Reporting live: Triplex formation is used to construct a stem-loop probe, which is opened upon binding of the DNA target (red). The triplex molecular beacon is composed of a single DNA strand (blue), a stem-forming oligomer (black), and is labeled with a fluorophore and a quencher (red and blue circles). This concept facilitates the introduction of further functionalities such as additional quenchers in the assembly of "superquenched" beacons. © 2007 Wiley-VCH Verlag GmbH & Co. KGaA.
引用
收藏
页码：5223 / 5225
页数：3
相关论文
共 50 条
  • [1] Quadruplex-based molecular beacons as tunable DNA probes
    Bourdoncle, A.
    Torres, A. Estevez
    Gosse, C.
    Lacroix, L.
    Vekhoff, P.
    Le Saux, T.
    Jullien, L.
    Mergny, J. -L.
    JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2006, 128 (34) : 11094 - 11105
  • [2] Quadruplex-based molecular beacons as tunable DNA probes
    Bourdoncle, A.
    Torres, A. Estévez
    Gosse, C.
    Lacroix, L.
    Vekhoff, P.
    Le Saux, T.
    Jullien, L.
    Mergny, J.-L.
    Journal of the American Chemical Society, 2006, 128 (34): : 11094 - 11105
  • [3] Detection of cancer cells using triplex DNA molecular beacons based on expression of enhanced green fluorescent protein (eGFP)
    Xi, Dongmei
    Wang, Xindong
    Ai, Shiyun
    Zhang, Shusheng
    CHEMICAL COMMUNICATIONS, 2014, 50 (67) : 9547 - 9549
  • [4] Molecular beacons - Novel fluorescent probes
    Fang, XH
    Li, JWJ
    Perlette, J
    Tan, WH
    Wang, KM
    ANALYTICAL CHEMISTRY, 2000, 72 (23) : 747A - 753A
  • [5] New hairpin-structured DNA probes:: alternatives to classical molecular beacons
    Friedrich, Achim
    Habl, Gregor
    Sauer, Markus
    Wolfrum, Juergen
    Hoheisel, Joerg
    Marme, Nicole
    Knemeyer, Jens-Peter
    ULTRASENSITIVE AND SINGLE-MOLECULE DETECTION TECHNOLOGIES II, 2007, 6444
  • [6] Classical Triplex Molecular Beacons for MicroRNA-21 and Vascular Endothelial Growth Factor Detection
    Lu, Shasha
    Wang, Shuang
    Zhao, Jiahui
    Sun, Jian
    Yang, Xiurong
    ACS SENSORS, 2018, 3 (11): : 2438 - 2445
  • [7] DNA nanomachines as evolved molecular Beacons for in vitro and in vivo detection
    Feng, Shuo
    Shang, Ye
    Wu, Fan
    Ding, Fei
    Li, Bin
    Xu, Jiahui
    Xu, Liang
    Zhou, Xiang
    TALANTA, 2014, 120 : 141 - 147
  • [8] Optical Fiber Nanotips Coated with Molecular Beacons for DNA Detection
    Giannetti, Ambra
    Barucci, Andrea
    Cosi, Franco
    Pelli, Stefano
    Tombelli, Sara
    Trono, Cosimo
    Baldini, Francesco
    SENSORS, 2015, 15 (05): : 9666 - 9680
  • [9] Detection of DNA methyltransferase activity using allosteric molecular beacons
    Zhang, Weiting
    Zu, Xiaolong
    Song, Yanling
    Zhu, Zhi
    Yang, Chaoyong James
    ANALYST, 2016, 141 (02) : 579 - 584
  • [10] Enzymatic signal amplification of molecular beacons for sensitive DNA detection
    Li, Jianwei Jeffery
    Chu, Yizhuo
    Lee, Benjamin Yi-Hung
    Xie, Xiaoliang Sunney
    NUCLEIC ACIDS RESEARCH, 2008, 36 (06)
12345